miR-137靶向作用RUNX2调控MC3T3-E1细胞成骨分化

目的探讨miR-137对RUNX2的靶向作用及其对MC3T3-E1细胞成骨分化的影响。方法通过软件预测miR-137与成骨标志基因RUNX2的结合位点。检测MC3T3-E1细胞诱导成骨分化过程中miR-137的表达变化。转染miR-137 mimics或miR-137 inhibitor后,再诱导MC3T3-E1细胞成骨分化,检测成骨分化标志物（RUNX2、ALP、OPN、OCN及OSX）表达变化。结果经软件预测,miR-137与靶基因RUNX2有结合位点。MC3T3-E1细胞成骨分化过程中,miR-137表达下调。转染miR-137mimics的MC3T3-E1细胞成骨标志基因表达受到抑制,...

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Published in中国骨质疏松杂志 Vol. 22; no. 11; pp. 1440 - 1445
Main Author 高翔 荆凯鹏 黄瑞 闫守泉 牛艳茹 李鹏 罗远明 楚佳奇
Format Journal Article
LanguageChinese
Published 广东医科大学附属医院干细胞研发与细胞治疗中心,广东湛江,524001%广东医科大学附属医院微创骨科研究室,广东湛江,524001%广东医科大学附属医院干细胞研发与细胞治疗中心,广东湛江524001 2016
广东医科大学附属医院微创骨科研究室,广东湛江524001
Subjects
MC3T3-E1细胞
miR-137
RUNX2
成骨分化
miR-137
成骨分化
MC3T3-E1细胞
Osteogenic differentiation
MC3T3-E1 cell
RUNX2
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ISSN1006-7108
DOI10.3969/j.issn.1006-7108.2016.11.016

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Summary:目的探讨miR-137对RUNX2的靶向作用及其对MC3T3-E1细胞成骨分化的影响。方法通过软件预测miR-137与成骨标志基因RUNX2的结合位点。检测MC3T3-E1细胞诱导成骨分化过程中miR-137的表达变化。转染miR-137 mimics或miR-137 inhibitor后,再诱导MC3T3-E1细胞成骨分化,检测成骨分化标志物（RUNX2、ALP、OPN、OCN及OSX）表达变化。结果经软件预测,miR-137与靶基因RUNX2有结合位点。MC3T3-E1细胞成骨分化过程中,miR-137表达下调。转染miR-137mimics的MC3T3-E1细胞成骨标志基因表达受到抑制,而转染miR-137 inhibitor组成骨标志基因呈不同程度表达上调。结论miR-137通过靶向作用RUNX2基因调控MC3T3-E1细胞成骨分化。
Bibliography:miR-137; RUNX2; MC3T3-E1 cell; Osteogenic differentiation
GAO Xiang1 , JING Kaipeng1 , HUANG Rui1 , YAN Shouquan1 , NIU Yanru2, LI Peng1 , LUO Yuanming1 , CHU Jiaqi1,2（ 1. Stem Cell Research and Cellular Therapy Center, The Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China ;2. Laboratory of Minimally Invasive Orthopedic Surgery, The Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China）
11-3701/R
Objective To investigate the targeting of RUNX2 by miR-137 and its effect on MC3T3-E1 cell differentiation.Me thods The binding site of miR-137 and osteogenic marker gene RUNX2 was predicted by using an online prediction program.The expression level of miR-137 in MC3T3-E1 cells was detected during osteogenic differentiation. After transfection with miR-137 or miR-137 inhibitor, the MC3T3-E1 cells were induced to differentiate into osteoblasts, and the expression of osteogenic differentiation markers（ RUNX2,ALP,OPN,OCN and OSX） were detected. Re sults The RUNX2 gene as a
ISSN:1006-7108
DOI:10.3969/j.issn.1006-7108.2016.11.016