Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice

• Published in: Plant and cell physiology Vol. 49; no. 10; pp. 1429 - 1450
• Main Authors: Hirano, K.(Nagoya Univ. (Japan). Bioscience and Biotechnology Center), Aya, K, Hobo, T, Sakakibara, H, Kojima, M, Shim, R.A, Hasegawa, Y, Ueguchi Tanaka, M, Matsuoka, M
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.10.2008; Oxford Publishing Limited (England)
• Subjects: ANTERA; Anther; ANTHERE; ANTHERS; Auxin; BIOSINTESIS; BIOSYNTHESE; BIOSYNTHESIS; Cluster Analysis; EXPRESION GENICA; EXPRESSION DES GENES; Gametogenesis - genetics; GENE EXPRESSION; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Genome, Plant; HIBRIDACION MOLECULAR; HYBRIDATION MOLECULAIRE; LASER; Laser microdissection; LASERS; Microarray; Microdissection - methods; MOLECULAR HYBRIDIZATION; Oligonucleotide Array Sequence Analysis - methods; Oryza - chemistry; Oryza - genetics; Oryza - growth & development; ORYZA SATIVA; Plant Growth Regulators - biosynthesis; Plant Growth Regulators - genetics; PLANT GROWTH SUBSTANCES; POLEN; POLLEN; Pollen - chemistry; Pollen - genetics; Pollen - growth & development; Rice; RNA, Plant - genetics; Sequence Alignment; Special Issue – Regular Papers; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; Anther; GA; Auxin; Microarray; Laser microdissection; Rice
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcn123

To investigate the involvement of phytohormones during rice microspore/pollen (MS/POL) development, endogenous levels of IAA, gibberellins (GAs), cytokinins (CKs) and abscisic acid (ABA) in the mature anther were analyzed. We also analyzed the global expression profiles of genes related to seven phytohormones, namely auxin, GAs, CKs, brassinosteroids, ethylene, ABA and jasmonic acids, in MS/POL and tapetum (TAP) using a 44K microarray combined with a laser microdissection technique (LM-array analysis). IAA and GA4 accumulated in a much higher amount in the mature anther compared with the other tissues, while CKs and ABA did not. LM-array analysis revealed that sets of genes required for IAA and GA synthesis were coordinately expressed during the later stages of MS/POL development, suggesting that these genes are responsible for the massive accumulation of IAA and GA4 in the mature anther. In contrast, genes for GA signaling were preferentially expressed during the early developmental stages of MS/POL and throughout TAP development, while their expression was down-regulated at the later stages of MS/POL development. In the case of auxin signaling genes, such mirror-imaged expression observed in GA synthesis and signaling genes was not observed. IAA receptor genes were mostly expressed during the late stages of MS/POL development, and various sets of AUX/IAA and ARF genes were expressed during the different stages of MS/POL or TAP development. Such cell type-specific expression profiles of phytohormone biosynthesis and signaling genes demonstrate the validity and importance of analyzing the expression of phytohormone-related genes in individual cell types independently of other cells/tissues.