Separated transcriptomes of male gametophyte and tapetum in rice: Validity of a laser microdissection (LM) microarray

• Published in: Plant and cell physiology Vol. 49; no. 10; pp. 1407 - 1416
• Main Authors: Suwabe, K.(Tohoku Univ., Sendai (Japan)), Suzuki, G, Takahashi, H, Shiono, K, Endo, M, Yano, K, Fujita, M, Masuko, H, Saito, H, Fujioka, T, Kaneko, F, Kazama, T, Mizuta, Y, Kawagishi Kobayashi, M, Tsutsumi, N, Kurata, N, Nakazono, M, Watanabe, M
• Format: Journal Article
• Language: English
• Published: Japan Oxford University Press 01.10.2008; Oxford Publishing Limited (England)
• Subjects: ANTERA; Anther; ANTHERE; ANTHERS; ARN; Cluster Analysis; EXPRESION GENICA; EXPRESSION DES GENES; Gametogenesis - genetics; GENE EXPRESSION; Gene Expression Profiling - methods; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Genome, Plant; HIBRIDACION MOLECULAR; HYBRIDATION MOLECULAIRE; L. Microspore/pollen; Laser microdissection; Lasers; Microarray; Microdissection - methods; MOLECULAR HYBRIDIZATION; Oligonucleotide Array Sequence Analysis - methods; Oryza - genetics; Oryza - growth & development; ORYZA SATIVA; POLEN; POLLEN; Pollen - genetics; Pollen - growth & development; RNA; RNA, Plant - genetics; Special Issue – Regular Papers; Tapetum; TRANSCRIPCION; TRANSCRIPTION; Anther; Tapetum; Microarray; L. Microspore/pollen; Laser microdissection
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcn124

In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.