Variovorax sp. Has an Optimum Cell Density to Fully Function as a Plant Growth Promoter

• Published in: Microorganisms (Basel) Vol. 7; no. 3; p. 82
• Main Authors: Natsagdorj, Oyungerel, Sakamoto, Hisayo, Santiago, Dennis Marvin O, Santiago, Christine D, Orikasa, Yoshitake, Okazaki, Kazuyuki, Ikeda, Seishi, Ohwada, Takuji
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.03.2019; MDPI
• Subjects: Agriculture; Bacteria; Biochemical tests; Biofilms; bioinoculant; Carboxylic acids; Cell density; Chinese cabbage; Clean technology; Colonization; Enzymes; Fisheries; GUS staining; Microorganisms; Plant growth; Plant growth promoters; Plant growth substances; plant growth-promoting bacteria; Plant roots; Roots; Seedlings; Siderophores; Sustainable agriculture; Variovorax; Vegetables; United States > US; Japan; Variovorax; GUS staining; plant growth-promoting bacteria; bioinoculant
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms7030082

Utilization of plant growth-promoting bacteria colonizing roots is environmentally friendly technology instead of using chemicals in agriculture, and understanding of the effects of their colonization modes in promoting plant growth is important for sustainable agriculture. We herein screened the six potential plant growth-promoting bacteria isolated from L. ( sp. HRRK 005, sp. HRRK 103, sp. HRRK 170, sp. HRRK 190, sp. HRTK 192, and sp. HRRK 193) using a series of biochemical tests. Among all strains screened, HRRK 170 had the highest potential for plant growth promotion, given its ability to produce plant growth substances and enzymes such as siderophores and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, respectively, concomitantly with active growth in a wider range of temperatures (10⁻30 °C) and pH (5.0⁻10.0). HRRK 170 colonized either as spots or widely on the root surface of all vegetable seedlings tested, but significant growth promotion occurred only in two vegetables (Chinese cabbage and green pepper) within a certain cell density range localized in the plant roots. The results indicate that HRRK 170 could function as a plant growth promoter, but has an optimum cell density for efficient use.