Lhx2 specifies regional fate in Emx1 lineage of telencephalic progenitors generating cerebral cortex
Cerebral cortex is comprised of regions, including six-layer neocortex and three-layer olfactory cortex, generated by telencephalic progenitors of an Emx1 lineage. The mechanism specifying region-specific subpopulations in this lineage is unknown. We found that the LIM homeodomain transcription fact...
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Published in | Nature neuroscience Vol. 12; no. 11; pp. 1381 - 1389 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.11.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Cerebral cortex is comprised of regions, including six-layer neocortex and three-layer olfactory cortex, generated by telencephalic progenitors of an Emx1 lineage. The mechanism specifying region-specific subpopulations in this lineage is unknown. We found that the LIM homeodomain transcription factor Lhx2 in mice, expressed in graded levels by progenitors, determines their regional identity and fate decisions to generate neocortex or olfactory cortex. Deletion of Lhx2 with Emx1-cre at embryonic day 10.5 (E10.5) altered the fates of progenitors, causing them to generate three-layer cortex, phenocopying olfactory cortex rather than lateral neocortex. Progenitors did not generate ectopic olfactory cortex following Lhx2 deletion at E11.5. Thus, Lhx2 regulates a regional-fate decision by telencephalic progenitors during a critical period that ends as they differentiate from neuroepithelial cells to neuronogenic radial glia. These findings establish a genetic mechanism for determining regional-fate in the Emx1 lineage of telencephalic progenitors that generate cerebral cortex. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 S-J.C.: Designed and generated the floxed allele of Lhx2, was a principal contributor to analysis of the Lhx2 conditional knockouts, prepared figures and assisted in writing the paper. C.P.G.: Principal contributor to the analysis of the Lhx2 conditional knockouts, prepared figures and assisted in writing the paper. All authors directly contributed to carrying out the study, have read the paper and agree with the findings. Specific contributions are as follows. T.K.: Contributed to the analysis of the Lhx2 conditional knockouts and assisted in preparing figures. D.O’L.: Conceived the study, designed and contributed to the analysis of the Lhx2 conditional knockouts, prepared figures and wrote the paper. Author contributions |
ISSN: | 1097-6256 1546-1726 |
DOI: | 10.1038/nn.2427 |