miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭
目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR...
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| Published in | 西安交通大学学报（医学版） Vol. 38; no. 2; pp. 226 - 230 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
华中科技大学同济医学院附属荆州医院肿瘤科,湖北荆州,433020%长江大学医学院,湖北荆州,433023%华中科技大学同济医学院附属荆州医院胸外科,湖北荆州,433020
2017
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
| DOI | 10.7652/jdyxb201702014 |
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| Abstract | 目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少（P〈0.05）,A549细胞的迁移侵袭减少（P〈0.01）;荧光素酶实验结果显示,miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性（P〈0.05）;与miR-498＋空质粒组比较,转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加（P〈0.05）,细胞的迁移侵袭增加（P〈0.01）。结论miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭。 |
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| AbstractList | 目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少（P〈0.05）,A549细胞的迁移侵袭减少（P〈0.01）;荧光素酶实验结果显示,miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性（P〈0.05）;与miR-498＋空质粒组比较,转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加（P〈0.05）,细胞的迁移侵袭增加（P〈0.01）。结论miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭。 R734.2; 目的 探索微小RNA498(miR-498)是否通过下调FOXM1抑制肺癌细胞系(A549)细胞的迁移和侵袭.方法 转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况.结果 与空质粒组比较,转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少(P<0.05),A549细胞的迁移侵袭减少(P<0.01);荧光素酶实验结果显示,miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性(P<0.05);与miR-498+空质粒组比较,转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加(P<0.05),细胞的迁移侵袭增加(P<0.01).结论 miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭. |
| Abstract_FL | Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by down-regulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion abilities of A549 cells were decreased in the miR-498 group (both P<0 .01 ).The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498 (P<0 .05 );over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells.Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1. |
| Author | 唐曦 胡娅 徐炎华 汪春林 邱萍 王向辉 |
| AuthorAffiliation | 华中科技大学同济医学院附属荆州医院肿瘤科,湖北荆州433020 长江大学医学院,湖北荆州433023 华中科技大学同济医学院附属荆州医院胸外科,湖北荆州433020 |
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| Author_FL | XU Yan-hua WANG Chun-lin TANG Xi HU Ya QIU Ping WANG Xiang-hui |
| Author_FL_xml | – sequence: 1 fullname: TANG Xi – sequence: 2 fullname: HU Ya – sequence: 3 fullname: XU Yan-hua – sequence: 4 fullname: WANG Chun-lin – sequence: 5 fullname: QIU Ping – sequence: 6 fullname: WANG Xiang-hui |
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| Keywords | COL1A1 miR-498 侵袭 COL1A5 invasion 微小RNA498(miR-498) lung adenocarcinoma FOXM1 肺腺癌 |
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| Notes | TANG Xi1 , HU Ya2 , XU Yan-hua1 , WANG Chun-lin1 , QIU Ping1 , WANG Xiang-hui3 （1. Department of Oncology, Jingzhou Hospital of Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 433020; 2. Medical College, Yangtze University, Jingzhou 433023; 3. Department of Cardiothoracic Surgery, Jingzhou Hospital of Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 433020, China） Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by downregulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion ab |
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| PublicationYear | 2017 |
| Publisher | 华中科技大学同济医学院附属荆州医院肿瘤科,湖北荆州,433020%长江大学医学院,湖北荆州,433023%华中科技大学同济医学院附属荆州医院胸外科,湖北荆州,433020 |
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| Snippet | 目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western... R734.2; 目的 探索微小RNA498(miR-498)是否通过下调FOXM1抑制肺癌细胞系(A549)细胞的迁移和侵袭.方法 转染miR-498mimic和miR-NC至培养的A549细胞中,Western... |
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| SubjectTerms | COL1A1 COL1A5 FOXM1 侵袭 微小RNA498（miR-498） 肺腺癌 |
| Title | miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭 |
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