miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭

目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR...

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Published in西安交通大学学报（医学版） Vol. 38; no. 2; pp. 226 - 230
Main Author 唐曦 胡娅 徐炎华 汪春林 邱萍 王向辉
Format Journal Article
LanguageChinese
Published 华中科技大学同济医学院附属荆州医院肿瘤科,湖北荆州,433020%长江大学医学院,湖北荆州,433023%华中科技大学同济医学院附属荆州医院胸外科,湖北荆州,433020 2017
Subjects
COL1A1
COL1A5
FOXM1
侵袭
微小RNA498（miR-498）
肺腺癌
COL1A1
miR-498
侵袭
COL1A5
invasion
微小RNA498(miR-498)
lung adenocarcinoma
FOXM1
肺腺癌
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ISSN1671-8259
DOI10.7652/jdyxb201702014

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Summary:目的探索微小RNA498（miR-498）是否通过下调FOXM1抑制肺癌细胞系（A549）细胞的迁移和侵袭。方法转染miR-498mimic和miR-NC至培养的A549细胞中,Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况,荧光素酶试验验证FOXM1是miR-498的靶基因;再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中,检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况,划痕试验和Transwell法观察细胞迁移侵袭情况。结果与空质粒组比较,转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少（P〈0.05）,A549细胞的迁移侵袭减少（P〈0.01）;荧光素酶实验结果显示,miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性（P〈0.05）;与miR-498＋空质粒组比较,转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加（P〈0.05）,细胞的迁移侵袭增加（P〈0.01）。结论miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭。
Bibliography:TANG Xi1 , HU Ya2 , XU Yan-hua1 , WANG Chun-lin1 , QIU Ping1 , WANG Xiang-hui3 （1. Department of Oncology, Jingzhou Hospital of Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 433020; 2. Medical College, Yangtze University, Jingzhou 433023; 3. Department of Cardiothoracic Surgery, Jingzhou Hospital of Tongji Medical College, Huazhong University of Science and Technology, Jingzhou 433020, China）
Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by downregulating FOXM1.Methods miR-498 mimic and miR-NC were transfected into A549 cells.Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells;Western blot was used to detect the expressions of COL1A1,COL1A5 and FOXM1 in A549 cells.Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498.Results Compared with those in the control group,the expressions of COL1A1,COL1A5 and FOXM1 were decreased,and the migration and invasion ab
ISSN:1671-8259
DOI:10.7652/jdyxb201702014