连续缝合间断打结吻合动脉在小鼠心脏移植中的应用

目的应用连续缝合间断打结法吻合动脉建立小鼠心脏移植模型。方法近交系C57BL/6j^（H-2b）、BALB/c^（H-2d）小鼠各30只,建立小鼠腹部心脏移植模型（C57BL/6j→BALB/c）,连续缝合间断打结法端侧吻合供体升主动脉和受体腹主动脉;一针后壁先缝法连续吻合供体肺动脉和受体下腔静脉。观察移植心脏搏动持续时间及受体生存时间。结果手术成功率90%（27/30）,总手术时间（91.77±6.70）min,动脉吻合时间（24.63±1.67）min,静脉吻合时间（15.17±1.80）min。供心中位存活时间8天,病理组织学示典型排斥反应。结论应用连续缝合间断打结法吻合动脉降低了小鼠心...

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Published in复旦学报（医学版） Vol. 43; no. 2; pp. 211 - 214
Main Author 崔得利 谭长军 刘忠
Format Journal Article
LanguageChinese
Published 大连医科大学附属第一医院普外科 大连116011%复旦大学附属中山医院肝癌研究所-上海市器官移植重点实验室 上海200032 2016
Subjects
动物模型
动脉吻合
小鼠
心脏移植
arterial anastomosis
mouse
心脏移植
heart transplantation
动物模型
小鼠
animal model
动脉吻合
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ISSN1672-8467
DOI10.3969/j.issn.1672-8467.2016.02.015

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Summary:目的应用连续缝合间断打结法吻合动脉建立小鼠心脏移植模型。方法近交系C57BL/6j^（H-2b）、BALB/c^（H-2d）小鼠各30只,建立小鼠腹部心脏移植模型（C57BL/6j→BALB/c）,连续缝合间断打结法端侧吻合供体升主动脉和受体腹主动脉;一针后壁先缝法连续吻合供体肺动脉和受体下腔静脉。观察移植心脏搏动持续时间及受体生存时间。结果手术成功率90%（27/30）,总手术时间（91.77±6.70）min,动脉吻合时间（24.63±1.67）min,静脉吻合时间（15.17±1.80）min。供心中位存活时间8天,病理组织学示典型排斥反应。结论应用连续缝合间断打结法吻合动脉降低了小鼠心脏移植的手术难度,效果可靠,值得推广。
Bibliography:CUI De-li, TAN Chang-jun, LIU Zhong （1. Department of General Surgery, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China; 2. Liver Cancer Institute, Zhongshan Hospital, Fudan University-Shanghai Key Laboratory of Organ Transplantation, Shanghai 200032, China）
mouse; animal model; heart transplantation; arterial anastomosis
31-1885/R
Objective To evaluate the feasibility of continuous-interrupted method for arterial anastomoses in mouse model of heart transplantation. Methods Heterotopic heart transplantations were performed from inbred C57BL/6j~（（H-2b））mouse（n=30）to BALB/c~（（H-2d））mouse（n=30）,in which the graft′s ascending aorta was anastomosed end-to-side to the recipient′s aorta by continuousinterrupted sutures,and the pulmonary artery was anastomosed end-to-side to the inferior vena cava by continuous one-suture anastomosis.The survival time of grafts and the duration of cardiac impulse were observed. Results The successful rate of transplantation was 90%（27/3
ISSN:1672-8467
DOI:10.3969/j.issn.1672-8467.2016.02.015