Fluorescence monitoring of rare circulating tumor cell and cluster dissemination in a multiple myeloma xenograft model in vivo

Circulating tumor cells (CTCs) are of great interest in cancer research because of their crucial role in hematogenous metastasis. We recently developed “diffuse in vivo flow cytometry” (DiFC), a preclinical research tool for enumerating extremely rare fluorescently labeled CTCs directly in vivo. In...

Full description

Saved in:
Bibliographic Details
Published inJournal of biomedical optics Vol. 24; no. 8; p. 085004
Main Authors Patil, Roshani, Tan, Xuefei, Bartosik, Peter, Detappe, Alexandre, Runnels, Judith M, Ghobrial, Irene, Lin, Charles P, Niedre, Mark
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.08.2019
S P I E - International Society for
Subjects
Animal models
Biopsy
Blood
Blood circulation
Blood vessels
Cancer
Enumeration
Flow cytometry
Fluorescence
Green fluorescent protein
Medical prognosis
Metastases
Metastasis
Multiple myeloma
Peripheral blood
Transgenic mice
Tumor cells
Tumors
Xenografts
Xenotransplantation
United States > US
New Jersey
multiple myeloma
optical devices
circulating tumor cells
fluorescence
circulating tumor cell clusters
Online AccessGet full text

Cover

More Information
Summary:Circulating tumor cells (CTCs) are of great interest in cancer research because of their crucial role in hematogenous metastasis. We recently developed “diffuse in vivo flow cytometry” (DiFC), a preclinical research tool for enumerating extremely rare fluorescently labeled CTCs directly in vivo. In this work, we developed a green fluorescent protein (GFP)-compatible version of DiFC and used it to noninvasively monitor tumor cell numbers in circulation in a multiple myeloma (MM) disseminated xenograft mouse model. We show that DiFC allowed enumeration of CTCs in individual mice overtime during MM growth, with sensitivity below 1  CTC mL  −  1 of peripheral blood. DiFC also revealed the presence of CTC clusters (CTCCs) in circulation to our knowledge for the first time in this model and allowed us to calculate CTCC size, frequency, and kinetics of shedding. We anticipate that the unique capabilities of DiFC will have many uses in preclinical study of metastasis, in particular, with a large number of GFP-expressing xenograft and transgenic mouse models.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors contributed equally to this work.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.24.8.085004