The role of oxidative stress-mediated apoptosis in the pathogenesis of uric acid nephropathy

• Published in: Renal failure Vol. 41; no. 1; pp. 616 - 622
• Main Authors: Yang, Lijuan, Chang, Baochao, Guo, Yaling, Wu, Xueping, Liu, Lei
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2019; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Animals; Apoptosis; Apoptosis Regulatory Proteins - metabolism; Bax protein; Caspase 3 - metabolism; Caspase 9 - metabolism; Caspase-9; Creatinine; Creatinine - blood; Epithelial cells; Glutathione; Glutathione - pharmacology; Immunoblotting; Kidney - physiopathology; Kidneys; Laboratory Study; Male; Malondialdehyde; Malondialdehyde - metabolism; Mitochondria; Mitochondria - pathology; Nephropathy; Oxidative Stress; p53 Protein; Random Allocation; Rats; Rats, Sprague-Dawley; reduced glutathione; Superoxide dismutase; Superoxide Dismutase - metabolism; Uric acid; Uric Acid - pharmacology; Uric acid nephropathy; Uric acid nephropathy; apoptosis; reduced glutathione; mitochondria; oxidative stress
• ISSN: 0886-022X; 1525-6049
• DOI: 10.1080/0886022X.2019.1633350

Objective: By copying the uric acid nephropathy rat model, the oxidative stress injury of mitochondria was caused in renal tubular epithelial cells and the relationship between the injury and the induction of cell apoptosis was identified. Methods: All rats were randomly divided into NC (normal control, NC) group, HUA (high uric acid, HUA) group and GSH (reductive glutathione, GSH) group. The values were quantitatively tested in the kidney tissues, including 24-h urinary protein quantity, serum creatinine, blood uric acid, the MDA (malondialdehyde, MDA) and SOD (superoxide dismutase, SOD) oxidative stress indicators. The expression of p53, Bax and caspase-9/-3 were detected by immunoblotting. TUNEL assays were used to detect the apoptosis of renal tubular epithelial cells. Result: In HUA and GSH groups, the 24-h urinary protein(24UTP), serum creatinine, and blood uric acid increased gradually with the increase of the replication cycle and the increase was significant compared to the NC group (p < .05). Compared to the NC group, MDA increased whereas SOD decreased. The expression of apoptotic proteins, such as p53, Bax, and caspase-9/-3 in the mitochondria was significantly different (p < .05). TUNEL assay revealed that the renal tubular epithelial cells in HUA group were largely apoptotic, whereas the GSH group improved significantly. Conclusion: Mitochondria incurred the substantial damage due to being in a state of oxidative stress, which was the primary cause of apoptosis in the renal tubule epithelial cells. GSH exhibited the effective resistance to the influence of oxidative stress and can restore the damage in the renal tubular epithelial cells.