The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR‐family regulator DasR and links N‐acetylglucosamine metabolism to the control of development

• Published in: Molecular microbiology Vol. 61; no. 5; pp. 1237 - 1251
• Main Authors: Rigali, Sébastien, Nothaft, Harald, Noens, Elke E. E., Schlicht, Maximilian, Colson, Sévrine, Müller, Marisa, Joris, Bernard, Koerten, Henk K., Hopwood, David A., Titgemeyer, Fritz, Van Wezel, Giles P.
• Format: Journal Article Web Resource
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2006; Blackwell Science; Blackwell Publishing
• Subjects: Acetylglucosamine - metabolism; Antibiotics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; Biological and medical sciences; Enzymes; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - genetics; Genes; Genetic Complementation Test; Genotype & phenotype; Glucosamine - analogs & derivatives; Glucosamine - pharmacology; Glucose-6-Phosphate - analogs & derivatives; Glucose-6-Phosphate - pharmacology; Life sciences; Metabolism; Microbiologie; Microbiology; Microorganisms; Microscopy, Electron, Scanning - methods; Miscellaneous; Models, Biological; Mutation - genetics; Phenotype; Phosphotransferases - genetics; Phosphotransferases - metabolism; Protein Binding - drug effects; Regulon - genetics; Sciences du vivant; Streptomyces; Streptomyces coelicolor; Streptomyces coelicolor - genetics; Streptomyces coelicolor - metabolism; Streptomyces coelicolor - ultrastructure; Substrate Specificity; Actinomycetales; Regulation(control); Isomerases; Streptomycetaceae; Microbiology; Enzyme; Streptomyces coelicolor; Intramolecular transferases; Bacteria; Actinomycetes; Metabolism; Phosphotransferases
• ISSN: 0950-382X; 1365-2958; 1365-2958
• DOI: 10.1111/j.1365-2958.2006.05319.x

Summary Members of the soil‐dwelling, sporulating prokaryotic genus Streptomyces are indispensable for the recycling of the most abundant polysaccharides on earth (cellulose and chitin), and produce a wide range of antibiotics and industrial enzymes. How do these organisms sense the nutritional state of the environment, and what controls the signal for the switch to antibiotic production and morphological development? Here we show that high extracellular concentrations of N‐acetylglucosamine, the monomer of chitin, prevent Streptomyces coelicolor progressing beyond the vegetative state, and that this effect is absent in a mutant defective of N‐acetylglucosamine transport. We provide evidence that the signal is transmitted through the GntR‐family regulator DasR, which controls the N‐acetylglucosamine regulon, including the pts genes ptsH, ptsI and crr needed for uptake of N‐acetylglucosamine. Deletion of dasR or the pts genes resulted in a bald phenotype. Binding of DasR to its target genes is abolished by glucosamine 6‐phosphate, a central molecule in N‐acetylglucosamine metabolism. Extracellular complementation experiments with many bld mutants showed that the dasR mutant is arrested at an early stage of the developmental programme, and does not fit in the previously described bld signalling cascade. Thus, for the first time we are able to directly link carbon (and nitrogen) metabolism to development, highlighting a novel type of metabolic regulator, which senses the nutritional state of the habitat, maintaining vegetative growth until changing circumstances trigger the switch to sporulation. Our work, and the model it suggests, provide new leads towards understanding how microorganisms time developmental commitment.