Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

Abstract The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains r...

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Published inNew microbes and new infections Vol. 2; no. 3; pp. 79 - 81
Main Authors Hansen, N, Rasmussen, A.K.I, Fiandaca, M.J, Kragh, K.N, Bjarnsholt, T, Høiby, N, Stender, H, Guardabassi, L
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.05.2014
Elsevier Limited
BlackWell Publishing Ltd
Elsevier
Subjects
Cystic fibrosis
identification
Infectious Disease
New Microbes in Humans – New Resistant Microbes in Humans
PNA FISH
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia
Cystic fibrosis
identification
PNA FISH
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Summary:Abstract The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 104 CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.
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Funding Information The research was supported by an unrestricted grant from the University of Copenhagen.
ISSN:2052-2975
2052-2975
DOI:10.1002/nmi2.38