亚麻MAPK基因克隆及盐碱胁迫下的表达分析
为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MP...
Saved in:
| Published in | 东北农业大学学报 Vol. 46; no. 3; pp. 21 - 28 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
黑龙江省农业科学院经济作物研究所,哈尔滨,150086%东北农业大学生命科学学院,哈尔滨,150030
2015
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | 为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MPK16在中性盐胁迫下上调表达,Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14在碱性盐胁迫下上调表达,Lu MPK16在碱性盐胁迫下下调表达。研究为亚麻MAPK基因功能,尤其是耐盐碱功能研究奠定理论基础。 |
|---|---|
| AbstractList | S563.2%Q785; 为研究MAPK基因与亚麻应答盐碱胁迫的关系，利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因，命名为LuMPK1～LuMPK20，这20个MAPK基因都含有T[D/E]Y保守结构域，除LuMPK5和LuMPK20以外，其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（LuMPK3、LuMPK8、LuMPK11、LuMPK14、LuMPK16），这5个基因序列与预测序列完全一致。利用数字基因表达谱数据，分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明，这5个基因的表达均受到盐碱胁迫影响。LuMPK3、LuMPK11、LuMPK14、LuMPK16在中性盐胁迫下上调表达，LuMPK3、LuMPK8、LuMPK11、LuMPK14在碱性盐胁迫下上调表达， LuMPK16在碱性盐胁迫下下调表达。研究为亚麻MAPK基因功能，尤其是耐盐碱功能研究奠定理论基础。 为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MPK16在中性盐胁迫下上调表达,Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14在碱性盐胁迫下上调表达,Lu MPK16在碱性盐胁迫下下调表达。研究为亚麻MAPK基因功能,尤其是耐盐碱功能研究奠定理论基础。 |
| Abstract_FL | In order to investigate the relationship between flax MAPK genes and saline-alkaline stress, 20 flax MAPK genes, named LuMPK1-LuMPK20, repectively, were predicted from flax genome by bioinformatics methods. These 20 MAPK genes al contained T[D/E]Y conserved domains and existed in pairs except LuMPK5 and LuMPK20. Five MAPK genes (LuMPK3, LuMPK8, LuMPK11, LuMPK14, LuMPK16) were cloned by PCR and their sequences were consistent with the predicted sequences. The expressions of these genes were analyzed by digital gene expression under saline and alkaline stresses. The results showed that the expressions of these five genes were subject to saline and alkaline stresses. LuMPK3, LuMPK11, LuMPK14, LuMPK16 were up-regulated under neutral salt stress, and LuMPK3, LuMPK8, LuMPK11, LuMPK14 were up-regulated under alkaline salt stress. LuMPK16 was down-regulated under alkaline salt stress. This study laid the theoretical foundation for the function of MAPK genes in flax, especial y the saline-alkaline tolerance function. |
| Author | 于莹 陈宏宇 程莉莉 赵东升 袁红梅 吴广文 关凤芝 |
| AuthorAffiliation | 黑龙江省农业科学院经济作物研究所,哈尔滨150086 东北农业大学生命科学学院,哈尔滨150030 |
| AuthorAffiliation_xml | – name: 黑龙江省农业科学院经济作物研究所,哈尔滨,150086%东北农业大学生命科学学院,哈尔滨,150030 |
| Author_FL | ZHAO Dongsheng CHEN Hongyu YUAN Hongmei GUAN Fengzhi WU Guangwen YU Ying CHENG Lili |
| Author_FL_xml | – sequence: 1 fullname: YU Ying – sequence: 2 fullname: CHEN Hongyu – sequence: 3 fullname: CHENG Lili – sequence: 4 fullname: ZHAO Dongsheng – sequence: 5 fullname: YUAN Hongmei – sequence: 6 fullname: WU Guangwen – sequence: 7 fullname: GUAN Fengzhi |
| Author_xml | – sequence: 1 fullname: 于莹 陈宏宇 程莉莉 赵东升 袁红梅 吴广文 关凤芝 |
| BookMark | eNotzT1Lw0AcBvAbKlhrv4SbQ-Du_pe73FiKb1jRoXu4S3I1oldtENtR1CJ2sHMLvk2Ck5tEqF-mMem3MFCnBx5-PM8aqtiujSqoSjB2HQlcrqJ6ksQaA2cgCBFVJObpZJF-HzSO9rPnNJu-ZHejxWSYPT7k03H-9lncXBc_H_OvUT65LV7fi9ksux_-Po3X0YpRp0lU_88aam9vtZu7TutwZ6_ZaDkBJ8xRgmvsUhJ5JooMDUNPhBIAyi5khAthKAGtA0k91xDNPEKZAQY0IEYpBVBDm8vZK2WNsh3_pHvZs-WhH2o7CPt9TTFxMWDMSruxtMFx13Yu4lKf9-Iz1Rv4nDMqJUgGf349YWA |
| ClassificationCodes | S563.2%Q785 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Cloning and expression analysis of MAPK gene under saline-alkaline stress in flax |
| DocumentTitle_FL | Cloning and expression analysis of MAPK gene under saline-alkaline stress in flax |
| EndPage | 28 |
| ExternalDocumentID | dbnydxxb201503004 664299394 |
| GrantInformation_xml | – fundername: 哈尔滨市青年科技创新人才专项; 黑龙江省农业科学院院级课题重点项目; 黑龙江省博士后资助经费项目 funderid: (2013RFQYJ011); (2012ZD011); (LBH-Z13182) |
| GroupedDBID | 2RA 92L ALMA_UNASSIGNED_HOLDINGS CDYEO CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| ID | FETCH-LOGICAL-c614-a76b0521e8feef2dd87d9333052d41677f213bbc9285f1b48124f3432c1faaa33 |
| ISSN | 1005-9369 |
| IngestDate | Wed Nov 06 04:24:43 EST 2024 Wed Feb 14 10:30:53 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Keywords | saline-alkaline stress flax gene cloning expression analysis 表达分析 亚麻 MAPK 盐碱胁迫 基因克隆 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c614-a76b0521e8feef2dd87d9333052d41677f213bbc9285f1b48124f3432c1faaa33 |
| Notes | MAPK;flax;MAPK;gene cloning;saline-alkaline stress;expression analysis 23-1391/S YU Ying, CHEN Hongyu, CHENG Lili, ZHAO Dongsheng, YUAN Hongmei, WU Guangwen, GUAN Fengzhi （1.Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 2. School of Life Sciences, Northeast Agricultural University, Harbin 150030, China） In order to investigate the relationship between flax MAPK genes and saline-alkaline stress, 20 flax MAPK genes, named LuMPK1-LuMPK20, repectively, were predicted from flax genome by bioinformatics methods. These 20 MAPK genes al contained T[D/E]Y conserved domains and existed in pairs except LuMPK5 and LuMPK20. Five MAPK genes （LuMPK3, LuMPK8, LuMPK11, LuMPK14, LuMPK16） were cloned by PCR and their sequences were consistent with the predicted sequences. The expressions of these genes were analyzed by digital gene expression under saline and alkaline stresses. The results showed that the expressions of these five genes were subject to saline and alkaline stres |
| PageCount | 8 |
| ParticipantIDs | wanfang_journals_dbnydxxb201503004 chongqing_primary_664299394 |
| PublicationCentury | 2000 |
| PublicationDate | 2015 |
| PublicationDateYYYYMMDD | 2015-01-01 |
| PublicationDate_xml | – year: 2015 text: 2015 |
| PublicationDecade | 2010 |
| PublicationTitle | 东北农业大学学报 |
| PublicationTitleAlternate | Journal of Northeast Agricultural University |
| PublicationTitle_FL | Journal of Northeast Agricultural University |
| PublicationYear | 2015 |
| Publisher | 黑龙江省农业科学院经济作物研究所,哈尔滨,150086%东北农业大学生命科学学院,哈尔滨,150030 |
| Publisher_xml | – name: 黑龙江省农业科学院经济作物研究所,哈尔滨,150086%东北农业大学生命科学学院,哈尔滨,150030 |
| SSID | ssib036437117 ssib012081041 ssib006567412 ssib001100945 ssib000272666 ssib002263419 ssib051368045 |
| Score | 2.0210505 |
| Snippet | 为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu... S563.2%Q785;... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 21 |
| SubjectTerms | MAPK 亚麻 基因克隆 盐碱胁迫 表达分析 |
| Title | 亚麻MAPK基因克隆及盐碱胁迫下的表达分析 |
| URI | http://lib.cqvip.com/qk/90164A/201503/664299394.html https://d.wanfangdata.com.cn/periodical/dbnydxxb201503004 |
| Volume | 46 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NaxNBFF9KT15EUbFWpYhzCpHdmdn5OM4mG4pS8ZBCPYXZ7G7rJfUjhdqbqEXswZ5T8OskePImEeo_05j07D_ge7PbdAMiKgzLy-ybj_febOY3s_veeN7NhNlUKR3UKU_DOqf4zGmbApBLwyRkuUzdQnHlrlhe5bfXwrW5uZ-Vr5a2-smt7s5v_Ur-x6qQB3ZFL9l_sOy0UsgAGuwLV7AwXP_KxiTmJDJEGxJrJKJoxdy7Q-KQ6Bb-RiIixkdCQYqQD7iVcDktooBHIo_2kTCURAGJFVEMECYSUYuYyDWjXHHpinO8ZQJilOOJMWGFytUsiI5JcTrnCewta9ANx9YgWjpCuJziluut4cS4W6ZJjKgQArtqwpPhUQquYtdZaF_XnGga-4ClYpSuJGTNyVYI4NiVnhI1J0GIaaaPzaKcQp2gKiS2BzT0BHPCmlOuTyKORKRRUyi5KMo5fUfMERLFwvbAUs3qLkvhYVpOCRirFY89rM4Z5bbpg-qWQjkBVKBE6fY-G89bCIQATGMMWw5_qQjk769W3wTTmSVlgB9_ViAtFTMh-ACPS34KUQMKGM8_hbgMX8xWfInDgAnlu7O7p4JhWJGNzd76IwBLznetl9veegVmtc95Z8v10ZIpBvt5b25n44Inj4aD4-E3HNyjd8PRwfvRy73jwe7ozevxwf7445fJ82eT75-Pvu6NBy8mHz5NDg9Hr3Z_vN2_6LVbcbuxXC9P_Kh3ASbWrRQJOpNnKs-ynKapkqlmDKYkmsLCQcqcBixJupqqMA8SjuA0R8_obpBbaxm75M33NnvZZW-Jh7lWGaW-SCxXFlb1UllOuZVBnneFXfAWpzJ3HhaBXTpT0yx4N0otdMrH_UknTXpP0-3tBEeHj3HqrvyxikXvDHIWu3VXvfn-463sGuDXfnLdGfwXNmZuiA |
| link.rule.ids | 315,783,787,4031 |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E4%BA%9A%E9%BA%BBMAPK%E5%9F%BA%E5%9B%A0%E5%85%8B%E9%9A%86%E5%8F%8A%E7%9B%90%E7%A2%B1%E8%83%81%E8%BF%AB%E4%B8%8B%E7%9A%84%E8%A1%A8%E8%BE%BE%E5%88%86%E6%9E%90&rft.jtitle=%E4%B8%9C%E5%8C%97%E5%86%9C%E4%B8%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5&rft.au=%E4%BA%8E%E8%8E%B9+%E9%99%88%E5%AE%8F%E5%AE%87+%E7%A8%8B%E8%8E%89%E8%8E%89+%E8%B5%B5%E4%B8%9C%E5%8D%87+%E8%A2%81%E7%BA%A2%E6%A2%85+%E5%90%B4%E5%B9%BF%E6%96%87+%E5%85%B3%E5%87%A4%E8%8A%9D&rft.date=2015&rft.issn=1005-9369&rft.volume=46&rft.issue=3&rft.spage=21&rft.epage=28&rft.externalDocID=664299394 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90164A%2F90164A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fdbnydxxb%2Fdbnydxxb.jpg |