亚麻MAPK基因克隆及盐碱胁迫下的表达分析

为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MP...

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Published in东北农业大学学报 Vol. 46; no. 3; pp. 21 - 28
Main Author 于莹 陈宏宇 程莉莉 赵东升 袁红梅 吴广文 关凤芝
Format Journal Article
LanguageChinese
Published 黑龙江省农业科学院经济作物研究所,哈尔滨,150086%东北农业大学生命科学学院,哈尔滨,150030 2015
Subjects
MAPK
亚麻
基因克隆
盐碱胁迫
表达分析
saline-alkaline stress
flax
gene cloning
expression analysis
表达分析
亚麻
MAPK
盐碱胁迫
基因克隆
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Summary:为研究MAPK基因与亚麻应答盐碱胁迫的关系,利用生物信息学方法从亚麻基因组中预测得到20个亚麻MAPK基因,命名为Lu MPK1~Lu MPK20,这20个MAPK基因都含有T[D/E]Y保守结构域,除Lu MPK5和Lu MPK20以外,其他基因都是两两一组。利用PCR技术克隆得到5个亚麻MAPK基因（Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14、Lu MPK16）,这5个基因序列与预测序列完全一致。利用数字基因表达谱数据,分析亚麻盐碱胁迫下这5个基因的表达情况。结果表明,这5个基因的表达均受到盐碱胁迫影响。Lu MPK3、Lu MPK11、Lu MPK14、Lu MPK16在中性盐胁迫下上调表达,Lu MPK3、Lu MPK8、Lu MPK11、Lu MPK14在碱性盐胁迫下上调表达,Lu MPK16在碱性盐胁迫下下调表达。研究为亚麻MAPK基因功能,尤其是耐盐碱功能研究奠定理论基础。
Bibliography:MAPK;flax;MAPK;gene cloning;saline-alkaline stress;expression analysis
23-1391/S
YU Ying, CHEN Hongyu, CHENG Lili, ZHAO Dongsheng, YUAN Hongmei, WU Guangwen, GUAN Fengzhi （1.Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 2. School of Life Sciences, Northeast Agricultural University, Harbin 150030, China）
In order to investigate the relationship between flax MAPK genes and saline-alkaline stress, 20 flax MAPK genes, named LuMPK1-LuMPK20, repectively, were predicted from flax genome by bioinformatics methods. These 20 MAPK genes al contained T[D/E]Y conserved domains and existed in pairs except LuMPK5 and LuMPK20. Five MAPK genes （LuMPK3, LuMPK8, LuMPK11, LuMPK14, LuMPK16） were cloned by PCR and their sequences were consistent with the predicted sequences. The expressions of these genes were analyzed by digital gene expression under saline and alkaline stresses. The results showed that the expressions of these five genes were subject to saline and alkaline stres
ISSN:1005-9369