Brassinosteroid-Regulated Gene Expression

• Published in: Plant physiology (Bethesda) Vol. 129; no. 3; pp. 1241 - 1251
• Main Authors: Carsten Müssig, Fischer, Sabine, Altmann, Thomas
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.07.2002; American Society of Plant Physiologists
• Subjects: alternative splicing; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis thaliana; Auxins; biochemical pathways; Biological and medical sciences; Brassinosteroids; Cell Biology and Signal Transduction; Cephalopelvic disproportion; Chemical agents; Cholestanols; Cholestanols - metabolism; Cholestanols - pharmacology; drug effects; Environmental conditions; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene expression regulation; Gene Expression Regulation, Plant; Gene Expression Regulation, Plant - drug effects; Genes; genetics; Genome, Plant; growth & development; metabolism; microarray technology; Molecular and cellular biology; Molecular genetics; mutants; Mutation; Oligonucleotide Array Sequence Analysis; pharmacology; Plant cells; plant development; Plant growth; Plant Growth Regulators; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Plant physiology and development; plant proteins; Plants; Protein synthesis; Signal Transduction; Signal Transduction - drug effects; Steroids, Heterocyclic; Steroids, Heterocyclic - metabolism; Steroids, Heterocyclic - pharmacology; transgenic plants; Vegetative apparatus, growth and morphogenesis. Senescence; Chromatin; Induction; Cell nucleus; Genetic variant; Functional deficit; Metabolic pathway; Gene expression; Structure function relationship; Cell wall; Stress; Dose activity relation; Arabidopsis thaliana; Brassinosteroid; Regulation(control); Cruciferae; Dicotyledones; Phytosterol; Angiospermae; Plant growth substance; Spermatophyta; Intraspecific comparison; Experimental plant
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.011003

Major brassinosteroid (BR) effects such as BR-induced growth are mediated through genomic pathways because RNA synthesis inhibitors and protein synthesis inhibitors interfere with these processes. A limited number of BR-regulated genes have been identified hitherto. The majority of genes (such as BRU1, CycD3, Lin6, OPR3, and TRIP-1) were identified by comparisons of BR-treated versus control-treated plants. However, altered transcript levels after BR application may not reflect normal physiological events. A complementary approach is the comparison of BR-deficient plants versus wild-type plants. No artificial treatments interfere with endogenous signaling pathways, but a subset of phenotypic alterations of phytohormone-deficient plants most probably is secondary. To identify genes that are subject to direct BR regulation, we analyzed CPD antisense and dwf1-6 (cbb1) mutant plants. Both show a mild phenotype in comparison with BR-deficient mutants such as cpd/cbb3, det2, and dwf4. Plants were grown under two different environments to filter out BR deficiency effects that occur only at certain environmental conditions. Finally, we established expression patterns after BR treatment of wild-type and dwf1-6 (cbb1) plants. Ideally, a BR-regulated gene displays a dose-response relationship in such a way that a gene with decreased transcript levels in BR-deficient plants is BR inducible and vice versa. Expression profile analysis of above ground part of plants was performed by means of Affymetrix Arabidopsis Genome Arrays.