Rat tooth pulp cells elicit neurite growth from trigeminal neurones and express mRNAs for neurotrophic factors in vitro

Molecular factors control the developmental ingrowth of axons to the tooth pulp. Here we examine the ability of pulpal cells to induce neurite outgrowth from neonatal rat trigeminal neurones (TGNs) in vitro. We found that TGNs emitted neurites and formed networks of branches in relation to pulpal ce...

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Bibliographic Details
Published inNeuroscience letters Vol. 308; no. 3; pp. 161 - 164
Main Authors Lillesaar, C., Eriksson, C., Fried, K.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 2001
Elsevier
Subjects
3T3 Cells
Animals
Basic Medicine
Biological and medical sciences
Cell culture
Cell physiology
Dental pulp
Dental Pulp - cytology
Dental Pulp - innervation
DNA Primers
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
In Vitro Techniques
Medical and Health Sciences
MEDICIN
Medicin och hälsovetenskap
MEDICINE
Medicinska och farmaceutiska grundvetenskaper
Mice
Molecular and cellular biology
Nerve Growth Factors - genetics
Neurites - physiology
Neuritogenesis
Neurosciences
Neurotrophic factors
Neurovetenskaper
Rat
Rats
Rats, Inbred F344
Rats, Sprague-Dawley
Responses to growth factors, tumor promotors, other factors
RNA, Messenger - analysis
Trigeminal ganglion
Trigeminal Nerve - cytology
Cell culture
Neuritogenesis
Rat
Trigeminal ganglion
Neurotrophic factors
Dental pulp
Peripheral nervous system
Neurotrophic factor
Vertebrata
Mammalia
Neurite
Growth
Rodentia
In vitro
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Summary:Molecular factors control the developmental ingrowth of axons to the tooth pulp. Here we examine the ability of pulpal cells to induce neurite outgrowth from neonatal rat trigeminal neurones (TGNs) in vitro. We found that TGNs emitted neurites and formed networks of branches in relation to pulpal cells. Neurones co-cultured with a mixture of pulpal cells and 3T3 fibroblasts formed networks exclusively in relation to the pulpal cells. Cultivated pulpal cells and pulpal tissue produced mRNAs for all neurotrophins and members of the glial cell line-derived neurotrophic factor family. Hence, rat pulpal cells have neuritogenic effects on single TGNs in vitro, that may be associated with secretion of neurotrophic factors.
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ISSN:0304-3940
1872-7972
1872-7972
DOI:10.1016/S0304-3940(01)01997-8