Comparison of prophylactic and therapeutic immunisation with an ErbB-2 (HER2) fusion protein and immunoglobulin V-gene repertoire analysis in a transgenic mouse model of spontaneous breast cancer

• Published in: Vaccine Vol. 32; no. 8; pp. 1012 - 1018
• Main Authors: Mukhopadhyay, Arunima, Dyring, Charlotte, Stott, David I
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 12.02.2014; Elsevier; Elsevier Limited
• Subjects: Allergy and Immunology; Animals; Antibodies, Neoplasm - blood; Antibody; Antibody Formation; Applied microbiology; B-Lymphocytes - immunology; Biological and medical sciences; Breast cancer; Cancer Vaccines - immunology; Epitopes, T-Lymphocyte - immunology; ErbB-2; Female; Fundamental and applied biological sciences. Psychology; Genes; Immunization; Immunoglobulin Variable Region - genetics; Kinases; Mammary Neoplasms, Experimental - immunology; Mammary Neoplasms, Experimental - prevention & control; Mammary Neoplasms, Experimental - therapy; Medical research; Medical sciences; Mice; Mice, Transgenic; Microbiology; Mouse model; Receptor, ErbB-2 - immunology; Recombinant Fusion Proteins - immunology; Repertoire; Rodents; Tolerance; Tumors; V-genes; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); ErbB-2; immunoglobulin; Antibody; H; Tolerance; Repertoire; Breast cancer; heavy; complementary DNA; V; variable; cDNA; Mouse model; V-genes; Ig; Animal model; Immunization; Immunoglobulins; Breast disease; Rodentia; Transgenic animal; Vaccine; Malignant tumor; Prevention; Mammary gland diseases; Vertebrata; Mammalia; Mouse; Immunologic repertoire; Fusion protein; Cancer
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2013.10.077

Highlights • Effectiveness of ErbB2-P30 fusion protein vaccine in transgenic mouse model of spontaneous development of breast cancer. • Breaking of self-tolerance with ErbB2-P30 vaccine and 100% protective antibody response after prophylactic vaccination. • Protective effect of vaccination but not therapeutic administration against endogenous mammary carcinoma in animal model. • Importance of spontaneous models of carcinoma compared with transplanted tumours in relation to human cancer. • Selection for particular Vκ and VH -gene family repertoire in antibody response.