Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis

The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The seque...

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Published inJournal of Bacteriology Vol. 175; no. 14; pp. 4383 - 4390
Main Authors Godon, J.J, Delorme, C, Bardowski, J, Chopin, M.C, Dusko Ehrlich, S, Renault, P
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.07.1993
Subjects
ACIDE AMINE
Amino Acid Sequence
Amino acids
Amino Acids, Branched-Chain - biosynthesis
AMINOACIDOS
Bacteriology
Base Sequence
BESOIN NUTRITIONNEL
Biological and medical sciences
Cloning, Molecular
CULTIVOS INICIADORES
CULTURE STARTER
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Escherichia coli - genetics
Evolution
Fundamental and applied biological sciences. Psychology
GENE
Gene Expression Regulation, Bacterial
GENES
Genes, Bacterial
Genetics
LACTOCOCCUS LACTIS
Lactococcus lactis - genetics
Lactococcus lactis - metabolism
LAIT FERMENTE
LECHE FERMENTADA
METABOLISME
METABOLISMO
Microbiology
Molecular Sequence Data
NECESIDADES DE NUTRIENTES
Nutrition
Plasmids
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
Transcription, Genetic
Transformation, Bacterial
Lactococcus lactis
Auxotrophy
Nucleotide sequence
Gene expression
Inactivation
Structure function relationship
Streptococcaceae
Regulation(control)
DNA
Bacteria
Micrococcales
Mutation
Aminoacid sequence
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Summary:The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the branched-chain amino acid auxotrophy. Nevertheless, the leu genes from the auxotroph appear to be transcribed and regulated similarly to those from the prototroph
Bibliography:9429225
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ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.175.14.4383-4390.1993