Sensitive Simultaneous Determinations of 1,2-Dihydroxynaphthalene and Catechol by an Amperometric Biosensor

• Published in: Analytical Sciences Vol. 37; no. 7; pp. 991 - 995
• Main Authors: GHOZALI, Ali A., KUMANO, Yusuke, TATSUMI, Hirosuke, ISWANTINI, Dyah, KUSMANA, Cecep, NURHIDAYAT, Novik
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10.07.2021; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Amperometry; Analytical Chemistry; Biosensing Techniques; Biosensors; Catechol; Catechols; Chemistry; Degradation; Electrical measurement; enzyme electrodes; Glucose 1-Dehydrogenase; Glucose dehydrogenase; Low concentrations; Metabolites; Naphthalene; Naphthols; Original Paper; Phenanthrene; Polycyclic aromatic hydrocarbons; Pyrroloquinoline quinone; Quinones; redox cycling; Redox properties; signal amplification; redox cycling; Biosensors; polycyclic aromatic hydrocarbons; signal amplification; enzyme electrodes
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.20P393

An amperometric biosensor for 1,2-dihydroxynaphthalene (DHN) and catechol (Cat) has been developed in order to monitor the biodegradaton of polycyclic aromatic hydrocarbons (PAHs). DHN is a common intermediary metabolite in naphthalene and phenanthrene degradation, while Cat is produced by further degradation. These compounds were detected by a biosensor modified with pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH). The biosensor was based on signal amplification by enzyme-catalyzed redox cycling and was able to detect DHN and Cat at very low concentrations down to 10−9 M. Since the anodic waves of DHN and Cat were well separated, simultaneous determinations of these compounds were possible. Although the current signal for DHN was reduced in repeated measurements due to the oxidative polymerization of DHN, it can be avoided when the concentration of DHN was sufficiently low (<1 μM).