Mapping molecular complexes with super-resolution microscopy and single-particle analysis

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular s...

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Bibliographic Details
Published inOpen biology Vol. 12; no. 7; p. 220079
Main Authors Mendes, Afonso, Heil, Hannah S, Coelho, Simao, Leterrier, Christophe, Henriques, Ricardo
Format Journal Article
LanguageEnglish
Published England Royal Society 01.07.2022
The Royal Society
Subjects
Life Sciences
Microscopy, Fluorescence - methods
Neurobiology
Neurons and Cognition
Review
single-particle analysis
structural biology
super-resolution microscopy
structural biology
super-resolution microscopy
single-particle analysis
biophysics/biotechnology/cellular biology/ structural biology structural biology
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Summary:Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.
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Special Feature: Advances in Quantitative Bioimaging. Guest edited by Ricardo Henriques, Ilaria Testa, Christophe Leterrier and Aubrey Weigel.
The authors contributed equally.
ISSN:2046-2441
2046-2441
DOI:10.1098/rsob.220079