Surface-Assisted Large-Scale Ordering of DNA Origami Tiles

• Published in: Angewandte Chemie International Edition Vol. 53; no. 29; pp. 7665 - 7668
• Main Authors: Aghebat Rafat, Ali, Pirzer, Tobias, Scheible, Max B., Kostina, Anna, Simmel, Friedrich C.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 14.07.2014; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Adhesion; Arrays; Cations; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA nanotechnology; DNA origami; Lattices; Mica; Microscopy, Atomic Force; Molecular structure; Nucleic Acid Conformation; self-assembly; Stacking; Static Electricity; Tiles; self-assembly; DNA nanotechnology; DNA origami
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201403965

The arrangement of DNA‐based nanostructures into extended higher order assemblies is an important step towards their utilization as functional molecular materials. We herein demonstrate that by electrostatically controlling the adhesion and mobility of DNA origami structures on mica surfaces by the simple addition of monovalent cations, large ordered 2D arrays of origami tiles can be generated. The lattices can be formed either by close‐packing of symmetric, non‐interacting DNA origami structures, or by utilizing blunt‐end stacking interactions between the origami units. The resulting crystalline lattices can be readily utilized as templates for the ordered arrangement of proteins. Molecular tessellation: Electrostatic control of adhesion and mobility of DNA origami structures on mica surfaces using monovalent cations facilitates formation of large, ordered 2D arrays of origami tiles. The lattices can be formed either by close‐packing of symmetric, non‐interacting DNA origami structures, or by utilizing blunt‐end stacking interactions between the origami units. The resulting crystalline lattices are readily utilized as templates for the ordered arrangement of proteins.