Detecting amplicons of loop-mediated isothermal amplification

Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time...

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Bibliographic Details
Published inMicrobiology and immunology Vol. 63; no. 10; p. 407
Main Author Shirato, Kazuya
Format Journal Article
LanguageEnglish
Published Australia 01.10.2019
Subjects
Bacterial Infections - microbiology
DNA, Bacterial - analysis
DNA, Protozoan - analysis
DNA, Viral - analysis
Fluorescence
Nephelometry and Turbidimetry - methods
Nucleic Acid Amplification Techniques - methods
Protozoan Infections - parasitology
Reproducibility of Results
Virus Diseases - virology
QPrimer
turbidity
detection
QProbe
LAMP
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Summary:Loop-mediated isothermal amplification (LAMP) assays are used to detect diverse pathogens. Initially, LAMP amplicons were detected using electrophoresis; later, real-time monitoring based on turbidity was developed to overcome the problem of contamination with environmental DNA. Recently, real-time monitoring of fluorescence signals using a quenching primer and probe has improved the reliability of amplification signals. Here, methods of detecting LAMP amplicons are reviewed.
ISSN:1348-0421
DOI:10.1111/1348-0421.12734