CaMKII反义寡核苷酸对阿尔茨海默病样大鼠海马细胞损伤的保护作用及其机制

摘要：目的研究CaMKII反义寡核苷酸对阿尔茨海默病（Alzheimer’s disease，AD）样大鼠海马细胞损伤的保护作用及其机制。方法采用Af31—40和AlCl3双干预法建立AD样大鼠模型；造模成功的大鼠随机分为AD模型组（AD）、溶剂对照组（TE）、反义寡核苷酸组（AS）和错义寡核苷酸组（MS）；以Morris水迷宫观察大鼠逃避潜伏期，用TUNEL染色检测大鼠海马细胞损伤情况；免疫印迹法检测CaMKII、NR1和NR2B在大鼠海马的表达变化。结杲AS组大鼠Morris水迷宫逃避潜伏期显著缩短，海马细胞损伤明显减轻，海马CaMKII、NR1和NR2B的表达显著减少；与AD组、TE组或...

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Published in西安交通大学学报（医学版） Vol. 38; no. 1; pp. 13 - 17
Main Author 赵小芳 钱冰 王静 吕春娥 宋远见 刘志安
Format Journal Article
LanguageChinese
Published 徐州医科大学解剖学教研室,江苏徐州,221004%杭州师范大学医学院,浙江杭州,311121%徐州卫生学校,江苏徐州,221400%徐州医科大学遗传学教研室,江苏徐州,221004 2017
Subjects
CaMKII
NR1
NR2B
大鼠
海马
阿尔茨海默病
NR2B
大鼠
CaMKII
Alzheimer’s disease
rat
hippocampus
海马
阿尔茨海默病
NR1
Online AccessGet full text
ISSN1671-8259
DOI10.7652/jdyxb201701003

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Summary:摘要：目的研究CaMKII反义寡核苷酸对阿尔茨海默病（Alzheimer’s disease，AD）样大鼠海马细胞损伤的保护作用及其机制。方法采用Af31—40和AlCl3双干预法建立AD样大鼠模型；造模成功的大鼠随机分为AD模型组（AD）、溶剂对照组（TE）、反义寡核苷酸组（AS）和错义寡核苷酸组（MS）；以Morris水迷宫观察大鼠逃避潜伏期，用TUNEL染色检测大鼠海马细胞损伤情况；免疫印迹法检测CaMKII、NR1和NR2B在大鼠海马的表达变化。结杲AS组大鼠Morris水迷宫逃避潜伏期显著缩短，海马细胞损伤明显减轻，海马CaMKII、NR1和NR2B的表达显著减少；与AD组、TE组或MS组相比，差异均具有统计学意义（P〈0．05）。结论CaMKII反义寡核苷酸能够通过改善AD样大鼠学习记忆能力和减轻AD样大鼠海马细胞损伤而发挥神经保护作用，且这种神经保护作用与其降低CaMKII、NR1和NR2B的病理性高表达有关。
Bibliography:ZHAO Xiao-fang , QIAN Bing , WANG Jing , LU Chun-e , SONG Yuan-jian , LIU Zhi-an （1. Department of Anatomy, Xuzhou Medical University, Xuzhou 221004; 2. Medical College, Hangzhou Normal University, Hangzhou 311121; 3. Xuzhou Health School, Xuzhou 221400; 4. Department of Genetics, Xuzhou Medical University, Xuzhou 221004, China）
CaMKII; NR1; NR2B; Alzheimer＇s disease; rat; hippocampus
Objective To study the protective effects of CaMKII antisense oligonucleotide on cell injury of rat hippocampus induced by Alzheimer＇s disease （AD） and its mechanism. Methods AD-like rat model was established by Aβ1-40 and AlCl3 intervention method. The model rats were then randomly divided into AD model group （AD）, solvent control group （TE）, CaMKII antisense oligonucleotide group （AS）, and CaMKII missense oligonucleotide group （MS）. The escape latency of the rats was observed by Morris water maze; the damage of hippocampal cells was detected by HE staining and TUNEL staining. The changes of CaMKII, NR1 and NR2B expressions in ra
ISSN:1671-8259
DOI:10.7652/jdyxb201701003