The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation

• Published in: Nature (London) Vol. 549; no. 7672; pp. 351 - 356
• Main Authors: Wallrapp, Antonia, Riesenfeld, Samantha J., Burkett, Patrick R., Abdulnour, Raja-Elie E., Nyman, Jackson, Dionne, Danielle, Hofree, Matan, Cuoco, Michael S., Rodman, Christopher, Farouq, Daneyal, Haas, Brian J., Tickle, Timothy L., Trombetta, John J., Baral, Pankaj, Klose, Christoph S. N., Mahlakõiv, Tanel, Artis, David, Rozenblatt-Rosen, Orit, Chiu, Isaac M., Levy, Bruce D., Kowalczyk, Monika S., Regev, Aviv, Kuchroo, Vijay K.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.09.2017; Nature Publishing Group
• Subjects: 14/63; 38/91; 631/250/2504/2506; 631/337/2019; 64/60; Allergens; Allergies; Allergy; Animals; Asthma; Cytokines; Female; Gene expression; Gene Expression Regulation; Gene sequencing; Generalized linear models; Genomics; Homeostasis; Humanities and Social Sciences; Hypersensitivity; Hypersensitivity - immunology; Hypersensitivity - pathology; Immunity, Innate - immunology; Inflammation; Inflammation - immunology; Inflammation - pathology; Interleukin-17 - immunology; Interleukin-33 - immunology; Ligands; Lung - immunology; Lung - pathology; Lung diseases; Lungs; Lymphocytes - immunology; Lymphoid cells; Male; Mice; Mice, Inbred C57BL; Mucosa; multidisciplinary; Neuromedin; Neuropeptides; Neuropeptides - metabolism; Physiological aspects; Receptors, Neurotransmitter - biosynthesis; Receptors, Neurotransmitter - genetics; Receptors, Neurotransmitter - metabolism; Respiratory Mucosa - immunology; Respiratory Mucosa - pathology; Ribonucleic acid; RNA; Science; Signal Transduction; Signaling; Stimulation; Subpopulations; Transcription activation; Transcription, Genetic; United States
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/nature24029

Type 2 innate lymphoid cells (ILC2s) both contribute to mucosal homeostasis and initiate pathologic inflammation in allergic asthma. However, the signals that direct ILC2s to promote homeostasis versus inflammation are unclear. To identify such molecular cues, we profiled mouse lung-resident ILCs using single-cell RNA sequencing at steady state and after in vivo stimulation with the alarmin cytokines IL-25 and IL-33. ILC2s were transcriptionally heterogeneous after activation, with subpopulations distinguished by expression of proliferative, homeostatic and effector genes. The neuropeptide receptor Nmur1 was preferentially expressed by ILC2s at steady state and after IL-25 stimulation. Neuromedin U (NMU), the ligand of NMUR1, activated ILC2s in vitro , and in vivo co-administration of NMU with IL-25 strongly amplified allergic inflammation. Loss of NMU–NMUR1 signalling reduced ILC2 frequency and effector function, and altered transcriptional programs following allergen challenge in vivo . Thus, NMUR1 signalling promotes inflammatory ILC2 responses, highlighting the importance of neuro-immune crosstalk in allergic inflammation at mucosal surfaces. Neuromedin receptor NMUR1 is specifically expressed by a subpopulation of type 2 innate lymphoid cells and promotes the inflammatory response of these cells in response to allergens, indicating the importance of neuro-immune crosstalk in allergic responses. Neuron regulation of immune cells Vijay Kuchroo and colleagues use single-cell RNA sequencing techniques to analyse the responses of lung innate lymphoid cells in mice to the epithelial-cell-derived cytokines IL-15 and IL-33. They identify the neuromedin U receptor NMUR1 as a receptor specifically expressed by a subpopulation of type 2 innate lymphoid cells (ILC2s), and show that it is activated by IL-25 plus the neuropeptide ligand neuromedin U (NMU), generating a lung inflammatory response. Loss of NMU–NMUR1 signalling results in allergic lung inflammation.