Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva

• Published in: Analytical and bioanalytical chemistry Vol. 413; no. 3; pp. 779 - 787
• Main Authors: Aspermair, Patrik, Mishyn, Vladyslav, Bintinger, Johannes, Happy, Henri, Bagga, Komal, Subramanian, Palaniappan, Knoll, Wolfgang, Boukherroub, Rabah, Szunerits, Sabine
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2021; Springer; Springer Nature B.V; Springer Verlag
• Subjects: 2D Nanomaterials for Electroanalysis; Analysis; Analytical Chemistry; Aptamers; Biochemistry; Biosensors; Carcinogenesis; Carcinogens; Characterization and Evaluation of Materials; Chemical Sciences; Chemistry; Chemistry and Materials Science; Confidence intervals; Disease transmission; E7 protein; Fabrication; Field effect transistors; Food Science; Graphene; Graphite; Human papillomavirus; Identification and classification; Laboratory Medicine; Methods; Monitoring/Environmental Analysis; Papillomavirus infections; Proteins; Pyrene; Research Paper; Ribonucleic acid; RNA; Saliva; Semiconductor devices; Technology assessment; Transistors; Belgium; Austria; Ireland; Surface functionalization; Point-of-care diagnostics; Field effect transistors; Reduced graphene oxide (rGO); Human papillomavirus (HPV); Aptamer; Electronic biosensor
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-020-02879-z

Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL −1 (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis. Graphical abstract