ELG1, a regulator of genome stability, has a role in telomere length regulation and in silencing
Telomeres, the natural ends of eukaryotic chromosomes, prevent the loss of chromosomal sequences and preclude their recognition as broken DNA. Telomere length is kept under strict boundaries by the action of various proteins, some with negative and others with positive effects on telomere length. Re...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 101; no. 6; pp. 1656 - 1661 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
10.02.2004
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Telomeres, the natural ends of eukaryotic chromosomes, prevent the loss of chromosomal sequences and preclude their recognition as broken DNA. Telomere length is kept under strict boundaries by the action of various proteins, some with negative and others with positive effects on telomere length. Recently, data have been accumulating to support a role for DNA replication in the control of telomere length, although through a currently poorly understood mechanism. Elg1p, a replication factor C (RFC)-like protein of yeast, contributes to genome stability through a putative replication-associated function. Here, we show that Elg1p participates in negative control of telomere length and in telomeric silencing through a replication-mediated pathway. We show that the telomeric function of Elg1 is independent of recombination and completely dependent on an active telomerase. Additionally, this function depends on yKu and DNA polymerase. We discuss alternative models to explain how Elg1p affects telomere length. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Deceased October 3, 2003. Communicated by Elizabeth Blackburn, University of California, San Francisco, CA, December 6, 2003 To whom correspondence should be addressed. E-mail: smolik@post.tau.ac.il. Abbreviations: Pol, polymerase; RFC, replication factor C; PCNA, proliferating cell nuclear antigen; YPD, yeast extract/peptone/dextrose; SD-Ura, synthetic dextrose medium lacking uracil. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0307796100 |