Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

• Published in: Journal of Genetic Engineering and Biotechnology Vol. 19; no. 1; pp. 50 - 8
• Main Authors: Bellaouchi, Reda, Abouloifa, Houssam, Rokni, Yahya, Hasnaoui, Amina, Ghabbour, Nabil, Hakkou, Abdelkader, Bechchari, Abdelmajid, Asehraou, Abdeslam
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 31.03.2021; Springer; Springer Nature B.V; Elsevier
• Subjects: Agricultural wastes; Amylases; Aspergillus niger; Biomass; Biomedical Engineering and Bioengineering; biotechnology; Byproducts; Carbon; carboxylic ester hydrolases; Cell culture; Cellulase; culture media; Date by-products; endo-1,4-beta-glucanase; Engineering; Enzymes; Extracellular; Extracellular enzymes; Fermentation; Floods; Hydrochloric acid; Optimization; pH effects; Potassium; Proteases; proteinases; quantitative analysis; Substrates; Morocco; Niger; India; France; United States > US; Enzymes; Extracellular; Date by-products; Aspergillus niger
• ISSN: 1687-157X; 2090-5920
• DOI: 10.1186/s43141-021-00145-y

Background This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products. Results The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation. Conclusion The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.