Next generation sequencing of pancreatic cyst fluid microRNAs from low grade-benign and high grade-invasive lesions

• Published in: Cancer letters Vol. 356; no. 2; pp. 404 - 409
• Main Authors: Wang, Jin, Paris, Pamela L, Chen, Jinyun, Ngo, Vy, Yao, Hui, Frazier, Marsha L, Killary, Ann M, Liu, Chang-Gong, Liang, Han, Mathy, Christian, Bondada, Sandhya, Kirkwood, Kimberly, Sen, Subrata
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 28.01.2015; Elsevier Limited
• Subjects: Adenocarcinoma, Mucinous - genetics; Adenocarcinoma, Mucinous - pathology; Biomarker; Biomarkers, Tumor - genetics; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - pathology; Cyst fluid; Cyst Fluid - metabolism; Cysts; Fluids; Gene Regulatory Networks; Hematology, Oncology and Palliative Medicine; High-Throughput Nucleotide Sequencing; Humans; IPMN; MicroRNAs - genetics; miRNAs; Morphology; Mortality; Mutation; Neoplasm Grading; Neoplasm Invasiveness; Pancreatic cancer; Pancreatic Cyst - genetics; Pancreatic Cyst - pathology; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Patients; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA polymerase; RNA, Messenger - genetics; Studies; Tumors; microRNAs; novel miRNAs associated with cancer; BC; pancreatic adenocarcinoma; DLBCL; Biomarker; miRNAs; diffuse large B cell lymphoma; Onc; non-small cell lung carcinoma; pancreatic ductal adenocarcinoma; tumor suppressor miR; OC; bladder cancer; UBC; Cyst fluid; miRNA; GC; rhabdomyosarcoma; hepatocellular carcinoma; quantitative real-time polymerase chain reaction; esophageal squamous cell carcinoma; Novel; gastric cancer; NSCLC; ovarian cancer; next generation sequencing; qRT-PCR; breast cancer; HCC; IPMN; intraductal papillary mucinous neoplasm; ESCC; PA; prostate cancer; renal cell carcinoma; PC; PDAC; RCC; Pancreatic cancer; RMS; oncogenic miR (oncomiR); NGS; urinary bladder cancer; TS; BC#
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2014.09.029

Abstract Intraductal papillary mucinous neoplasm (IPMN) is a precursor cystic lesion to pancreatic cancer. With the goal of classifying IPMN cases by risk of progression to pancreatic cancer, we undertook an exploratory next generation sequencing (NGS) based profiling study of miRNAs (miRNome) in the cyst fluids from low grade-benign and high grade-invasive pancreatic cystic lesions. Thirteen miRNAs (miR-138, miR-195, miR-204, miR-216a, miR-217, miR-218, miR-802, miR-155, miR-214, miR-26a, miR-30b, miR-31, and miR-125) were enriched and two miRNAs (miR-451a and miR-4284) were depleted in the cyst fluids derived from invasive carcinomas. Quantitative real-time polymerase chain reaction analysis confirmed that the relative abundance of tumor suppressor miR-216a and miR-217 varied significantly in these cyst fluid samples. Ingenuity Pathway Analysis (IPA) analysis indicated that the genes targeted by the differentially enriched cyst fluid miRNAs are involved in five canonical signaling pathways, including molecular mechanisms of cancer and signaling pathways implicated in colorectal, ovarian and prostate cancers. Our findings make a compelling case for undertaking in-depth analyses of cyst fluid miRNomes for developing informative early detection biomarkers of pancreatic cancer developing from pancreatic cystic lesions.