The PSI-E subunit of photosystem I binds ferredoxin:NADP + oxidoreductase

• Published in: FEBS letters Vol. 311; no. 2; pp. 169 - 173
• Main Authors: Andersen, Birgitte, Scheller, Henrik Vibe, Møller, Birger Lindberg
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.10.1992; Elsevier
• Subjects: 3,3′-dithiobis(sulfosuccinimidylpropionate); 4-morpholine-ethanesulphonic acid; Biological and medical sciences; Cell structures and functions; chl; chlorophyll; Chloroplast, photosynthetic membrane and photosynthesis; Cross-linking; Cross-Linking Reagents; DM-PS I; DTSSP; EDC; Electron Transport; Ethyldimethylaminopropyl Carbodiimide - pharmacology; Ferredoxin-NADP Reductase - metabolism; Ferredoxin:NADP + oxidoreductase; FNR; FNR-binding protein; Fundamental and applied biological sciences. Psychology; Hordeum - chemistry; LHC I; Light; light-harvesting complex I; MES; Molecular and cellular biology; Molecular Weight; N-[2-hydroxy-1,I-bis(hydromethyl)-ethyl]glycine; n-decyl-β-d-maltopyranoside; N-ethyl-3-[3-(dimethylamino)propyl]carbodiimide; Photosynthetic Reaction Center Complex Proteins - isolation & purification; Photosynthetic Reaction Center Complex Proteins - metabolism; Photosystem I; Photosystem I Protein Complex; Plastocyanin - metabolism; PS I; PS I purified with n-decyl-β-d-maltopyranoside; PS I purified with Triton X-100; PSI-E; SDS; sodium dodecyl sulfate; Succinimides - pharmacology; Tricine; TX-PS I; LHC I; TX-PS I; Tricine; SDS; DTSSP; EDC; chl; Photosystem I; DM; PS I; FNR; PSI-E; Cross-linking; FNR-binding protein; MES; DM-PS I; Ferredoxin:NADP + oxidoreductase; Photosystem 1; Monocotyledones; Ferredoxin-NADP+ reductase; Hordeum vulgare; Enzyme; Gramineae; Angiospermae; Electron transfer; Molecular interaction; Spermatophyta; Subunit
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81391-X

A photosystem I complex containing the polypeptides PSI-A to PSI-L, light-harvesting complex I and ferredoxin:NADP + oxidoreductase has been isolated from barley using the non-ionic detergent n-decyl-β- d-maltopyranoside. The ratio between bound forredoxin:NADP + oxidoreductase and P700 is 0.4 ± 0.2. The complex is highly active in catalyzing light-induced transfer of electrons from plastocyanin to NADP + at rates of 280±150 and 1800 ± 800, μmol NADPH/(mg chl h), without and in the presence of saturating amounts of exogenously added ferredoxin:NADP + oxidoreductase, respectively. Endogenously bound ferredoxin:NADP + oxidoreductase interacts with the PSI-E subunit as demonstrated by cross-linking experiments using two different types of cross-linkers and identification of the products by Western blotting and the use of monospecific antibodies.