Inhibition by Bacterial Lipopolysaccharide of Spontaneous and TNF-α-Induced Human Neutrophil Apoptosis In Vitro

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 39; no. 9; pp. 715 - 723
• Main Authors: Hachiya, Osamu, Takeda, Yuji, Miyata, Hiroaki, Watanabe, Hiroshi, Yamashita, Takao, Sendo, Fujiro
• Format: Journal Article
• Language: English
• Published: Tokyo Blackwell Publishing Ltd 01.01.1995; Center For Academic Publications Japan; Center for Academic Publications Japan
• Subjects: Ageing, cell death; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Biological and medical sciences; Cell physiology; Cell Survival; Cells, Cultured; Cycloheximide - pharmacology; DNA - analysis; DNA - drug effects; DNA Damage - drug effects; Dose-Response Relationship, Drug; Electrophoresis, Agar Gel; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Humans; Lipopolysaccharides - pharmacology; LPS; Molecular and cellular biology; Neutrophil; Neutrophils - drug effects; Neutrophils - physiology; Neutrophils - ultrastructure; Protein Synthesis Inhibitors - pharmacology; TNF-α; Tumor Necrosis Factor-alpha - pharmacology; Cell culture; Cell death; Immunomodulation; Cytokine; Lipopolysaccharide; Bacteria; Neutrophil; Tumor necrosis factor α; Apoptosis
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1995.tb03247.x

In the previous paper (Takeda et al, Int. Immunol., 5, 691-694, 1993), we demonstrated that tumor necrosis factor-α (TNF-α) promptly accelerates apoptosis of human neutrophils in vitro. In order to determine the role of neutrophil apoptosis in defending against bacterial infection, we studied the effect of bacterial lipopolysaccharide (LPS) on this process. LPS inhibited spontaneous and TNF-α-induced human neutrophil apoptosis in vitro, as determined by 1) light and electron microscopy, 2) flow cytometry, and 3) agarose gel electrophoresis of DNA. Low concentrations of cycloheximide, a protein synthesis inhibitor, which alone did not affect neutrophil apoptosis, were able to reduce spontaneous apoptosis inhibition by LPS, suggesting the involvement of newly synthesized protein in this phenomenon.