叶酸挽救乙醇致斑马鱼胚胎心脏和流出道发育异常

目的观察乙醇对斑马鱼胚胎心脏和流出道发育的干扰作用,并初步探讨叶酸挽救乙醇所致心脏和流出道发育异常的最佳时段和可能机制。方法在斑马鱼胚胎发育不同时段予以乙醇处理,观察胚胎的发育异常情况。将受精后7~12 h被予以乙醇组定义为乙醇处理组。在乙醇处理组胚胎的发育不同时段给予外源性叶酸进行叶酸挽救实验,观察各叶酸干预组胚胎的发育状况,探寻叶酸挽救的最佳时段。将受精后7~12 h给予叶酸组定义为叶酸挽救组。观察并统计乙醇处理组以及叶酸挽救组的胚胎发育异常百分比、存活百分比、心脏和流出道形态及其异常百分比、心率、心室收缩指数等指标来评估胚胎的发育状况。利用荧光显微造影的方法探查心脏流出道形态,采用胚胎整...

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Bibliographic Details
Published in复旦学报（医学版） Vol. 43; no. 2; pp. 135 - 141
Main Author 孙淑娜 姚泽忠 蒋璆 桂永浩
Format Journal Article
LanguageChinese
Published 上海市闵行区中心医院儿科 上海201199%上海市闵行区中心医院儿科 上海201199%上海市心血管病研究所 上海200032%复旦大学附属儿科医院心内科 上海201102 2016
复旦大学附属儿科医院心内科 上海201102
Subjects
bmp2b
tbx1
乙醇
叶酸
心脏
斑马鱼
流出道
心脏
斑马鱼
tbx1
乙醇
叶酸
zebrafish
folic acid
bmp2b
ethanol
流出道
heart
outflow tract
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ISSN1672-8467
DOI10.3969/j.issn.1672-8467.2016.02.002

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Summary:目的观察乙醇对斑马鱼胚胎心脏和流出道发育的干扰作用,并初步探讨叶酸挽救乙醇所致心脏和流出道发育异常的最佳时段和可能机制。方法在斑马鱼胚胎发育不同时段予以乙醇处理,观察胚胎的发育异常情况。将受精后7~12 h被予以乙醇组定义为乙醇处理组。在乙醇处理组胚胎的发育不同时段给予外源性叶酸进行叶酸挽救实验,观察各叶酸干预组胚胎的发育状况,探寻叶酸挽救的最佳时段。将受精后7~12 h给予叶酸组定义为叶酸挽救组。观察并统计乙醇处理组以及叶酸挽救组的胚胎发育异常百分比、存活百分比、心脏和流出道形态及其异常百分比、心率、心室收缩指数等指标来评估胚胎的发育状况。利用荧光显微造影的方法探查心脏流出道形态,采用胚胎整体原位杂交和Real-time PCR方法检测胚胎bmp2b、tbx1的表达情况。结果乙醇可干扰胚胎发育,乙醇处理组胚胎存在明显心脏和流出道形态异常以及心功能损害,在胚胎发育早期乙醇的干扰作用最明显。给予外源性叶酸能挽救乙醇导致的胚胎发育异常,在胚胎受精后7~12 h叶酸挽救作用最显著。乙醇处理组胚胎心脏和流出道的bmp2b、tbx1表达下降;予以叶酸干预后,乙醇处理组胚胎bmp2b和tbx1表达水平上调。结论叶酸能有效挽救乙醇导致的心脏和流出道发育异常,其机制可能是上调乙醇处理组胚胎bmp2b、tbx1的表达水平。
Bibliography:SUN Shu-na ,YAO Ze-zhong ,JIANG Qiu ,GUI Yong-hao （1. Department of Cardiology, Children＇s Hospital of Fudan University, Shanghai 201102, China ; 2. Department of Pediatrics, Central Hospital of Minhang District, Shanghai 201199, China ; 3. Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China）
Objective To observe ethanol-induced cardiac phenotypes and identify ethanol-sensitive stages during embryonic development,and to explore the optimal period during which the administration of folic acid can effectively rescue the ethanol-induced heart and outflow tract（OFT）defects and its underlying mechanisms. Methods Ethanol was administrated to zebrafish embryos in a series of developmental stages（7-12 h）and the abnormal heart and OFT phenotypes were observed after ethanol exposure.The embryos which were given ethanol at 7-12 hpf was defined as ethanol treated group.Administrating folic acid to ethanol-treated zebrafish embryos in different stages（7-12h）was conducted to rescue the abnormal cardiac def
ISSN:1672-8467
DOI:10.3969/j.issn.1672-8467.2016.02.002