经方青娥丸对去势大鼠骨质疏松作用机制的实验研究

目的探讨经方青娥丸治疗雌性大鼠卵巢切除后所致骨质疏松的作用机制。方法采用卵巢切除术建立绝经后骨质疏松大鼠模型,建模8周后,分别进行药物干预12周后,双能X线法测定各组大鼠的骨密度、骨组织形态计量学测定骨微结构变化、ELISA检测血清中骨代谢标志物BALP、CTX-1表达情况、以及雌激素E2水平变化。结果 1.骨密度：与治疗前比较,OVX组股骨及椎骨骨密度值均显著降低;雌激素组治疗后股骨和椎骨骨密度值较治疗前均有明显升高;青娥丸组治疗后股骨和椎骨骨密度值也均有一定程度升高。2.骨组织形态计量学：与OVX组比较,QEW组、E2组TBV%均显著升高,TRS%、TFS%、MAR、m AR和OSW均显著...

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Published in中国骨质疏松杂志 Vol. 23; no. 2; pp. 197 - 202
Main Author 王晓燕 常时新 李冠武 周自明 袁军 王乐 宋彦颖 缪宇
Format Journal Article
LanguageChinese
Published 上海中医药大学附属岳阳中西医结合医院,上海200437 2017
中国中医科学院广安门医院,北京100053%上海中医药大学附属岳阳中西医结合医院,上海,200437
Subjects
中医中药
绝经后骨质疏松症
雌激素样作用
青娥丸
骨代谢
骨微结构
Estrogenic effects
Chinese medicine
骨微结构
绝经后骨质疏松症
青娥丸
Bone microarchitecture,Bone metabolism
骨代谢
雌激素样作用
中医中药
Postmenopausal osteoporosis
Qing'E formula
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Summary:目的探讨经方青娥丸治疗雌性大鼠卵巢切除后所致骨质疏松的作用机制。方法采用卵巢切除术建立绝经后骨质疏松大鼠模型,建模8周后,分别进行药物干预12周后,双能X线法测定各组大鼠的骨密度、骨组织形态计量学测定骨微结构变化、ELISA检测血清中骨代谢标志物BALP、CTX-1表达情况、以及雌激素E2水平变化。结果 1.骨密度：与治疗前比较,OVX组股骨及椎骨骨密度值均显著降低;雌激素组治疗后股骨和椎骨骨密度值较治疗前均有明显升高;青娥丸组治疗后股骨和椎骨骨密度值也均有一定程度升高。2.骨组织形态计量学：与OVX组比较,QEW组、E2组TBV%均显著升高,TRS%、TFS%、MAR、m AR和OSW均显著降低。与SHAM组比较,QEW组和E2组TBV%均显著降低,TRS%、TFS%均显著增高;QEW组MAR显著增高,E2组则无明显差异;E2组m AR和OSW均显著增高,而QEW组则无明显差异。3.血清骨代谢生化标志物水平：E2组和QEW组BALP水平较OVX组均显著升高、CTX水平均显著降低,QEW组CTX水平显著高于E2组。4.雌激素水平：E2组和QEW组雌激素水平较OVX组均显著升高,而QEW组雌激素水平显著低于E2组。结论青娥丸治疗绝经后大鼠骨质疏松症可能与改善骨微结构、调节骨代谢、雌激素样作用相关。
Bibliography:Objective The current study aimed to explore the possible mechanisms that could contribute to the effects of Qing’ E formula on postmenopausal osteoporosis（PMOP） by establishing a mouse model featuring PMOP.Methods Postmenopausal osteoporosis was established by using ovariectomyinduced rat model.8 weeks after modeling,each group received drug therapy for12 weeks.Dual X-ray absorptiometry was used to measure bone mineral density,bone tissue morphology metrology to determine the bone micro structure changes,ELISA to detect the bone metabolic markers,BALP and CTX-L,expression levels and estrogen E2 level changes in serum.Results 1.BMD：compared with before the treatment,the lumbar spine and femur BMD values decreased significantly in the OVX group,increased significantly in the E2 group,and increased but did not reach significance level in the Qing’E Formula group.2.Bone histomorphometry parameters：The TBV%in E2 group and QEW group were significantly higher and TRS%,TFS%,MAR,mAR and OSW were significantly lower t
ISSN:1006-7108
DOI:10.3969/j.issn.1006-7108.2017.02.012