Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

• Published in: Regenerative therapy Vol. 16; pp. 63 - 72
• Main Authors: Su, Xin, Wang, Ting, Guo, Shu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2021; Japanese Society for Regenerative Medicine; Elsevier
• Subjects: 3D printing; Bone tissue engineering; Review; Scaffold materials; Stem cells; LIPUS; PRF; rBMSCs; SLA; STL; MSCs; SLM; HAp; ESCs; dECM; CHMA; CAD; AM; iPS; BCP; DCM; CAP; Stem cells; PCL; BMSCs; TCP; hMSCs; hADSC; PPSU; Scaffold materials; pcHμPs; PDA; SF-BG; FDM; MBG/SA–SA; ABS; LDM; PLLA; ASCs; SCAPs; PEGDA; GO; RAD16-I; Bone tissue engineering; HTy; ECM; PED; 3D; CT; PC; PEG; PLGA; HA; PVA; Alg; 3D printing; RAD16-I, a soft nanofibrous self-assembling peptide; GO, graphene oxide; MBG/SA–SA, mesoporous bioactive glass/sodium alginate-sodium alginate; ECM, extracellular matrix; LIPUS, low intensity pulsed ultrasound; HTy, 4-hydroxyphenethyl 2-(4-hydroxyphenyl) acetate; hMSCs, human mesenchymal stem cells; SLA, Stereolithography; HAp, hydroxyapatite nanoparticles; PLLA, poly l-lactide; iPS, induced pluripotent stem; ASCs, adult stem cells; dECM, decellularized bovine cartilage extracellular matrix; pcHμPs, novel self-healable pre-cross- linked hydrogel microparticles; SLM, Selective Laser Melting; ABS, Acrylonitrile Butadiene Styrene plastic; AM, additive manufacturing; PLGA, poly (lactide-co-glycolide); 3D, three-dimensional; CAD, computer-aided design; Alg, alginate; BMSCs, bone marrow-derived mesenchymal stem cells; PCL, polycraprolactone; CAP, cold atmospheric plasma; HA, hydroxyapatite; SCAPs, human stem cells from the apical papilla; SF-BG, silk fibroin and silk fibroin-bioactive glass; CHMA, chitosan methacrylate; FDM, fused deposition molding; CT, computed tomography; DCM, dichloromethane; TCP, β-tricalcium phosphate; PED, Precision Extrusion Deposition; STL, standard tessellation language; PEG, Polyethylene glycol; rBMSCs, rat bone marrow stem cells; PC, Polycarbonate; MSCs, Marrow stem cells; BCP, biphasic calcium phosphate; PDA, polydopamine; PVA, polyvinyl alcohol; PRF, platelet-rich fibrin; hADSC, human adipose derived stem cells; PEGDA, poly (ethylene glycol) diacrylate; LDM, Low Temperature Deposition Modeling; ESCs, embryonic stem cells; PPSU, Polyphenylene sulfone resins
• ISSN: 2352-3204; 2352-3204
• DOI: 10.1016/j.reth.2021.01.007

Due to traffic accidents, injuries, burns, congenital malformations and other reasons, a large number of patients with tissue or organ defects need urgent treatment every year. The shortage of donors, graft rejection and other problems cause a deficient supply for organ and tissue replacement, repair and regeneration of patients, so regenerative medicine came into being. Stem cell therapy plays an important role in the field of regenerative medicine, but it is difficult to fill large tissue defects by injection alone. The scientists combine three-dimensional (3D) printed bone tissue engineering scaffolds with stem cells to achieve the desired effect. These scaffolds can mimic the extracellular matrix (ECM), bone and cartilage, and eventually form functional tissues or organs by providing structural support and promoting attachment, proliferation and differentiation. This paper mainly discussed the applications of 3D printed bone tissue engineering scaffolds in stem cell regenerative medicine. The application examples of different 3D printing technologies and different raw materials are introduced and compared. Then we discuss the superiority of 3D printing technology over traditional methods, put forward some problems and limitations, and look forward to the future.