Spatiotemporal changes of tissue glycans depending on localization in cardiac aging

• Published in: Regenerative therapy Vol. 22; pp. 68 - 78
• Main Authors: Itakura, Yoko, Hasegawa, Yasuko, Kikkawa, Yurika, Murakami, Yuina, Sugiura, Kosuke, Nagai-Okatani, Chiaki, Sasaki, Norihiko, Umemura, Mariko, Takahashi, Yuji, Kimura, Tohru, Kuno, Atsushi, Ishiwata, Toshiyuki, Toyoda, Masashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2023; Japanese Society for Regenerative Medicine; Elsevier
• Subjects: Aging; Cardiac tissue; Glycan profile; Lectin microarray; Microvessels; Original; VVA; PHA-L; Lectin microarray; DBA; PNA; RCA120; LTL; TJA-I; SNA; STL; α-SMA; FITC; GSL-I; SBA; UDA; WFA; Aging; Microvessels; GlcNAc; Calsepa; PBS; SSA; ECA; Glycan profile; ACG; DAPI; EMT; ECM; GalNAc; MAL-I; PCA; WGA; Cardiac tissue; LEL; Gal; MAH; Man; HE; NPA; BPL; ConA; MAH, Maackia amurensis hemagglutinin; STL, Solanum tuberosum lectin; HE, hematoxylin-eosin; PNA, Arachis hypogaea agglutinin; GSL-I, Griffonia simplicifolia lectin I; PCA, principal component analysis; VVA, Vicia villosa agglutinin; PHA-L, Phaseolus vulgaris leucoagglutinin; UDA, Urtica dioica; PBS, phosphate-buffered saline; WGA, Triticum vulgaris agglutinin (wheat germ agglutinin); Gal, galactose; LEL, Lycopersicon esculentum lectin; SBA, Glycine max agglutinin; RCA120, Ricinus communis agglutinin I; Man, mannose; GalNAc, N-acetylgalactosamine; FITC, fluorescein isothiocyanate; ECA, Erythrina cristagalli agglutinin; ConA, Canavalia ensiformis lectin; DAPI, 4′,6-diamidino-2-phenylindole; BPL, Bauhinia purpurea alba lectin; DBA, Dolichos biflorus agglutinin; ECM, extracellular matrices; MAL-I, Maackia amurensis lectin I; WFA, Wisteria floribunda agglutinin; α-SMA, alpha smooth muscle actin; GlcNAc, N-acetylglucosamine; LTL, Lotus tetragonolobus lectin; SSA, Sambucus sieboldiana agglutinin; TJA-I, Trichosanthes japonica agglutinin I; SNA, Sambucus nigra agglutinin; EMT, endothelial-to-mesenchymal transition; ACG, Agrocybe cylindracea galectin; Calsepa, Calystegia sepium agglutinin; NPA, Narcissus pseudonarcissus agglutinin
• ISSN: 2352-3204; 2352-3204
• DOI: 10.1016/j.reth.2022.12.009

Heart failure is caused by various factors, making the underlying pathogenic mechanisms difficult to identify. Since cardiovascular disease tends to worsen over time, early diagnosis is key for treatment. In addition, understanding the qualitative changes in the heart associated with aging, where information on the direct influences of aging on cardiovascular disease is limited, would also be useful for treatment and diagnosis. To fill these research gaps, the focus of our study was to detect the structural and functional molecular changes associated with the heart over time, with a focus on glycans, which reflect the type and state of cells. Methods: We investigated glycan localization in the cardiac tissue of normal mice and their alterations during aging, using evanescent-field fluorescence-assisted lectin microarray, a technique based on lectin-glycan interaction, and lectin staining. Results: The glycan profiles in the left ventricle showed differences between the luminal side (medial) and wall side (lateral) regions. The medial region was characterized by the presence of sialic acid residues. Moreover, age-related changes in glycan profiles were observed at a younger age in the medial region. The difference in the age-related decrease in the level of α-galactose stained with Griffonia simplicifolia lectin-IB4 in different regions of the left ventricle suggests spatiotemporal changes in the number of microvessels. Conclusions: The glycan profile, which retains diverse glycan structures, is supported by many cell populations, and maintains cardiac function. With further research, glycan localization and changes have the potential to be developed as a marker of the signs of heart failure. [Display omitted] Glycan profiles differ in each region of the cardiac tissue.•Glycan levels changed in the respective cardiac tissue regions during aging.•These alterations affect the microvessels in heart tissues at differing rates.•Age-related changes in glycan profiles associated with each heart region are minor.