A Novel Spermatogenesis Related Factor-2 (SRF-2) Gene Expression Affected by TCDD Treatment

• Published in: Endocrine Journal Vol. 52; no. 1; pp. 75 - 81
• Main Authors: YAMANO, Yoshiaki, OHYAMA, Kenji, OHTA, Masanori, SANO, Tomoaki, RITANI, Akishige, SHIMADA, Junichi, ASHIDA, Nobuhisa, YOSHIDA, Emi, IKEHARA, Kiyomi, MORISHIMA, Isao
• Format: Journal Article
• Language: English
• Published: Japan The Japan Endocrine Society 01.02.2005
• Subjects: Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Amino Acid Sequence; Animals; DNA, Complementary - metabolism; Endocrine disruptor; Epididymis - drug effects; Gene Expression - drug effects; Gene Library; Genetic Testing; In situ hybridization; Male; Molecular Sequence Data; Organ Size - drug effects; Polychlorinated Dibenzodioxins - pharmacology; RabGAP/TBC protein; Rats; Rats, Sprague-Dawley; Sperm Count; Spermatogenesis; TCDD; Testis - anatomy & histology; Testis - metabolism; Time Factors; Tissue Distribution
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.52.75

We have cloned a gene which is specifically expressed at the stage of sexual maturation in the rat testis by means of differential display, and have named it spermatogenesis-related factor-2 (SRF-2). Testicular expression was first detected at 5 weeks of age, and its level of the expression increased up to 7 weeks, and was maintained even at 63 weeks. Its cDNA was 2,789 bp in length and encoded an open reading frame of 718 amino acids. This gene was mainly expressed in the spermatocyte, judging from the result of in situ hybridization. The hypothetical gene product had a motif highly homologous with RabGAP/TBC protein. Taken together, this gene is considered to have some important functions for meiosis. The gene expression was significantly decreased by treatment with TCDD, a candidate endocrine disruptor, when administered to male rats of the nursling period. Body weight and testis weight were decreased by the treatment, but even then the sperm concentration in cauda epididymis was not changed significantly. SRF-2 gene may be a promising biomarker to construct a detection system of uncertain endocrine disruptors.