Effect of Citrate on Production of Diacetyl and Acetoin by Lactococcus lactis ssp. lactis CNRZ 483 Cultivated in the Presence of Oxygen

• Published in: Journal of dairy science Vol. 80; no. 4; pp. 634 - 639
• Main Authors: Boumerdassi, H., Monnet, C., Desmazeaud, M., Corrieu, G.
• Format: Journal Article
• Language: English
• Published: Savoy, IL Elsevier Inc 01.04.1997; Am Dairy Sci Assoc; American Dairy Science Association
• Subjects: absorbance; absorbancia; acetic acid; acetoin; acetoina; acetoine; acide acetique; acide lactique; acido acetico; acido lactico; Agricultural sciences; Biological and medical sciences; Biotechnology; citrate; citrates; citratos; culture media; diacetilo; diacetyl; diacetyle; Food industries; Fundamental and applied biological sciences. Psychology; lactic acid; lactococcus lactis; Lactococcus lactis ssp. lactis CNRZ 483; lactoserum; lactosuero; Life Sciences; medio de cultivo; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; milieu de culture; Milk and cheese industries. Ice creams; oxigeno; oxygen; oxygene; presion; pression; pressure; rendement; rendimiento; whey; yields; Lactococcus lactis ssp. lactis CNRZ 483; acetoin; citrate; diacetyl; Lactic acid bacteria; 2-Butanone(3-hydroxy); Citrate; Dairy product; Environmental factor; Volatile organic compound; Fermented product; Biacetyl; Streptococcaceae; Lactococcus lactis subsp. lactis; Biotransformation; Concentration effect; Production; Microorganism culture; Bacteria; Micrococcales; Application; BIOTECHNOLOGIE; LACTOCOCCUS LACTIS
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.S0022-0302(97)75981-2

The effect was studied of trisodium citrate addition on the growth and formation of diacetyl and acetoin by Lactococcus lactis ssp. lactis CNRZ 483 in a whey-based medium under an oxygen pressure of 2atm. Growth increased, as shown by the final absorbance of the culture, which was 1.2 in the absence of citrate and 2.8 in the presence of 26mM citrate. In the absence of citrate, maximal concentrations of diacetyl and acetoin were 0.09 and 0.29mM, respectively. When citrate was added at 6.2, 11.5, and 26mM, the maximal diacetyl concentrations were 0.45, 0.53, and 0.85mM, and those of acetoin were 3.6, 4.5, and 8.5mM, respectively. However, under these conditions, the yield of citrate bioconversion to diacetyl and acetoin decreased as the initial concentration of citrate increased. Yields were 121, 83, and 70% in the presence of 6.2, 11.5, and 26mM citrate, respectively.