In vivo Multiphoton Microscopy Technique to Reveal the Physiology of the Mouse Placenta
Problem Pregnancy is a challenge to the maternal immune system as it must defend the body against pathogens while at the same time develop immune tolerance against the fetus growing inside the uterus. Despite ex vivo techniques being used to understand these processes, in vivo techniques are missing...
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| Published in | American journal of reproductive immunology (1989) Vol. 68; no. 3; pp. 271 - 278 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Denmark
Blackwell Publishing Ltd
01.09.2012
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Problem
Pregnancy is a challenge to the maternal immune system as it must defend the body against pathogens while at the same time develop immune tolerance against the fetus growing inside the uterus. Despite ex vivo techniques being used to understand these processes, in vivo techniques are missing.
Method of Study
To directly study these phenomena, we have developed a new microscope stage and surgical procedures for use in two‐photon microscopy, for in vivo observation of the mouse placenta.
Results
These tools and surgical procedures demonstrate fetal and maternal blood flow inside the labyrinth zone of the placenta, as well as its three dimensional structure. It was also useful to identify Plasmodium chabaudi‐infected red blood cells inside this labyrinth zone.
Conclusion
We believe this technique will represent an important contribution for expanding the available knowledge concerning cell dynamics and interactions at the fetal‐maternal interface. |
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| Bibliography: | ark:/67375/WNG-RFFFT73S-4 Video S1. Fetal blood flow inside the placenta.Video S2. 3D reconstruction of maternal-fetal blood vessels inside the placenta.Video S3. 3D reconstruction of placenta.Video S4. Maternal blood flow inside the placenta.Video S5. Plasmodium chabaudi-infected red blood cells (Pc-iRBCs) stop flowing inside the placenta. Figure S1. Representation of mouse placenta and Plasmodium chabaudi-GFP. This diagram provides a clear understanding of the entire structure of the mouse placenta labyrinth area. (a) A representation of the placenta in a lateral/cross section view; the fetus, umbilical cord, labyrinth zone, and deciduas area, where the maternal blood vessels (red) in this cross section view of the labyrinth area are cut transversally. (b) A top view of the same labyrinth area where the maternal (red) and fetal (green) blood vessels can be seen in a longitudinal section; which is the view provided in our placenta movies. (c) P. chabaudi expressing GFP infects red blood cells (RBCs) and these infected RBCs can be identified within the placenta by GFP expression (green); red: DAPI staining; blue: CFP expression by fetal tissues; white bar =10 μm; the arrow shows one GFP-iRBC. istex:E94FB9F0F63038899EDF6C9E4C7BFF32240B6A78 ArticleID:AJI1161 http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms Re‐use of this article is permitted in accordance with the Terms and Conditions set out at ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms Citation Zenclussen AC, Olivieri DN, Dustin ML, Tadokoro CE. In vivo multiphoton microscopy technique to reveal the physiology of the mouse placenta. Am J Reprod Immunol 2012; 68: 271–278 |
| ISSN: | 1046-7408 1600-0897 |
| DOI: | 10.1111/j.1600-0897.2012.01161.x |