Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用
目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达。结果诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组。结论脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通...
Saved in:
| Published in | 西安交通大学学报（医学版） Vol. 38; no. 6; pp. 839 - 843 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
西安交通大学第一附属医院骨科,陕西西安,710061%西安市第四医院超声诊断科,陕西西安,710004
2017
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1671-8259 |
| DOI | 10.7652/jdyxb201706011 |
Cover
| Abstract | 目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达。结果诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组。结论脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通路,分化过程可能受不同信号通路的共同调控。 |
|---|---|
| AbstractList | 目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达。结果诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组。结论脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通路,分化过程可能受不同信号通路的共同调控。 R651.2; 目的 探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用.方法 第3代脂肪干细胞分为3组:诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10 d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达.结果 诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组.结论 脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通路,分化过程可能受不同信号通路的共同调控. |
| Abstract_FL | Objective To investigate the role of Wnt/β-catenin signaling pathway in the process of adipose-derived stem cells (ADSCs)differentiating into neurons.Methods The third generation of ADSCs were divided into three groups.Neural induction medium was used in induction group and DDK-1 was added into neural induction medium in inhibition group.Normal culture medium was used in control group.Ten days after culture,Real-time PCR and Western blot were used to detect the expressions of NSE,β-catenin and GSK-3βin each group.Results The expressions of NSE and β-catenin were high but the expression of GSK-3 was low in induction group.The expression ofβ-catenin was lower but GSK-3 was higher in inhibition group;the expression of NSE was much lower than that in induction group,but higher than control group.Conclusion The differentiation of ADSCs into neurons is related to activation of Wnt signaling pathway,but Wnt signal pathway is not the only pathway to regulate ADSCs differentiation which may be controlled by different signaling pathways. |
| Author | 姬文晨 蒋婉婷 冯娇 李萌 |
| AuthorAffiliation | 西安交通大学第一附属医院骨科,陕西西安710061 西安市第四医院超声诊断科,陕西西安710004 |
| AuthorAffiliation_xml | – name: 西安交通大学第一附属医院骨科,陕西西安,710061%西安市第四医院超声诊断科,陕西西安,710004 |
| Author_FL | JI Wen-chen JIANG Wan-ting LI Meng FENG Jiao |
| Author_FL_xml | – sequence: 1 fullname: JI Wen-chen – sequence: 2 fullname: JIANG Wan-ting – sequence: 3 fullname: FENG Jiao – sequence: 4 fullname: LI Meng |
| Author_xml | – sequence: 1 fullname: 姬文晨 蒋婉婷 冯娇 李萌 |
| BookMark | eNpNkM9KAkEAxudgkJnXHqBbh82Znd2Z2WNI_0Cog9BRZnZnba3GUiO9hZoFBRZEBxGhQyBBBF4sJHyDnsJht7dowQ6dvsuP7_fxLYGEKisJwAqC65TYZqbkNerChIhCAhFKgCQiFBnMtJ1FkK5WAwGRyYiDiZUE-weqlvkeGS6vSRWo2fRZd8c_l71o_K77w6jdjJqv-nMUTjpRa6DvH8KXQTjp6quWvunou6doeh0Ob2cfb2GvPfvqh4_DZbDg8-OqTP9lCuS3NvPZHSO3t72b3cgZLkHIoBb2ocDUFdhhkgokhSVcj9u-IMwWHpOCISodCgWMd1LCISGE-8ixpe-YDKfA2rz2giufq2KhVD6vqFhYqPPGkVf_d0DMrs5Z97CsimdBTJ9WghNeaRQIxRazqc3wL0LMdts |
| ClassificationCodes | R651.2 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W92 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.7652/jdyxb201706011 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-工程技术 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | The role of Wnt/β-catenin signaling pathway in the process of adipose-derived stem cells differentiating into neurons |
| DocumentTitle_FL | The role of Wnt/β-catenin signaling pathway in the process of adipose-derived stem cells differentiating into neurons |
| EndPage | 843 |
| ExternalDocumentID | xaykdxxb201706011 673485758 |
| GrantInformation_xml | – fundername: 西安市科技计划项目(2016050SF/YX06-1); 西安交通大学第一附属医院院基金 Supported by Science and Technology Foundation of Xi'an(2016050SF/YX06-1); the Foundation of the First Affiliated Hospi-tal of Xi'an Jiaotong University |
| GroupedDBID | --- -05 2RA 5XA 5XF 7X7 8FI 8FJ 92L ABDBF ABUWG AFKRA ALMA_UNASSIGNED_HOLDINGS BENPR BPHCQ BVBZV BVXVI CCEZO CCPQU CIEJG CQIGP EOJEC FYUFA HMCUK OBODZ PQQKQ PROAC U1G U5O UKHRP W92 ~WA 2B. 4A8 92I 93N ACUHS ALIPV PSX TCJ |
| ID | FETCH-LOGICAL-c611-743f0b37cb398e7b1eb4bcda5fb685bd8eb817e970b036476a0666af195ef9283 |
| ISSN | 1671-8259 |
| IngestDate | Thu May 29 03:54:45 EDT 2025 Wed Feb 14 09:56:23 EST 2024 |
| IsPeerReviewed | false |
| IsScholarly | true |
| Issue | 6 |
| Keywords | 脂肪干细胞 differentiation Wnt/β-catenin signaling pathway Wnt/β-catenin信号通路 神经元 adipose-derived stem cell neuron 分化 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c611-743f0b37cb398e7b1eb4bcda5fb685bd8eb817e970b036476a0666af195ef9283 |
| Notes | JI Wen-chen1 , JIANG Wan-ting2 , FENG Jiao1 , LI Meng1 （1.Department of Orthopedics, the First Affiliated Hospital of Xi＇an Jiaotong University, Xi＇an 710061; 2 Department of Ultrasound Diagnosis, the Fourth Hospital of Xi＇an, Xi＇an 710004, China） Wnt/β-catenin signaling pathway; adipose-derived stem cell; neuron; differentiation Objective To investigate the role of Wnt/β-catenin signaling pathway in the process of adiposederived stem cells（ADSCs）differentiating into neurons.Methods The third generation of ADSCs were divided into three groups.Neural induction medium was used in induction group and DDK-1 was added into neural induction medium in inhibition group.Normal culture medium was used in control group.Ten days after culture,Real-time PCR and Western blot were used to detect the expressions of NSE,β-catenin and GSK-3βin each group.ResultsThe expressions of NSE andβ-catenin were high but the expression of GSK-3 was low in induction group.The expression ofβ-catenin was lower but GSK-3 was higher in inhibiti |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_xaykdxxb201706011 chongqing_primary_673485758 |
| PublicationCentury | 2000 |
| PublicationDate | 2017 |
| PublicationDateYYYYMMDD | 2017-01-01 |
| PublicationDate_xml | – year: 2017 text: 2017 |
| PublicationDecade | 2010 |
| PublicationTitle | 西安交通大学学报（医学版） |
| PublicationTitleAlternate | Journal of Xi‘an Jiaotong University：Medical Sciences |
| PublicationTitle_FL | Journal of Xi'an Jiaotong University(Medical Sciences) |
| PublicationYear | 2017 |
| Publisher | 西安交通大学第一附属医院骨科,陕西西安,710061%西安市第四医院超声诊断科,陕西西安,710004 |
| Publisher_xml | – name: 西安交通大学第一附属医院骨科,陕西西安,710061%西安市第四医院超声诊断科,陕西西安,710004 |
| SSID | ssib012869364 ssib038076391 ssib001051245 ssib022315977 ssib001104403 ssib002263349 ssib036440245 ssib051375640 ssib002039773 ssib006705401 ssib012416230 ssj0001537995 ssib052002713 |
| Score | 2.0770197 |
| Snippet | 目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western... R651.2; 目的 探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用.方法 第3代脂肪干细胞分为3组:诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10 d,利用Real-time PCR和Western... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 839 |
| SubjectTerms | Wnt/β-catenin信号通路 分化 神经元 脂肪干细胞 |
| Title | Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用 |
| URI | http://lib.cqvip.com/qk/90540A/201706/673485758.html https://d.wanfangdata.com.cn/periodical/xaykdxxb201706011 |
| Volume | 38 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LaxRBEB5ivHgRRcUYlSD2cc28-nXsmZ0lCIqHiLmFmZ2ZBIWNjwSSnCSJUVCIgngIIeBBCIIIuUQJkn_gr8iy67-wqnuy2zE5xMCy1HRV13xT1Ttd1TtT7Ti3RZOXYerKWlGWaS3MsrQm8xR3c_cFLTnM2frf83v32djD8O4EnRgY3LSeWpqbze40F499r-Q0XoU28Cu-Jfsfnu0phQagwb_wDR6G7xP5-BEWDWiQOCGRX8NHm1qQ5ichiRpEeSShRDRIxEki9fMMiiQCD1UDWTImSmCLgGjS14RPlEJWJEEfSTiJIiKYZgVEJroX6PGQpahuMTJaoaAohoTQvYCIidTdAY_guhewIo1QEFXHFkAFALCljpCwJQQxO2ZGDXC6SJ9FJURILa-ICq1Lo3iouCbqRDGLYEQo1JCANWKEZ7AB8r4MR7XIMjK95REtAsBjVANXg9cBhDzAqEUEkb6-MJCVGp8mIm5rAZsYy6MRLA5oqxORaC0u4LLXYcwLp_o3c1oraOwIXxsdxEAY4KPRQZtX-bUyh2YpHwkYCjg-OGoDl_txxZXUQnIEkh9jHScG8vQ4wFRj8C1UMIwi_ByDAcYNw1GF3QMSuVphA31pXGEG0CngmZ2oq6mYca8m_KpefDVXB8K6J9kTrzAlsQ5iOFPM-N_wgDOK5YYf5wvzmW8KR1Uz_eGS6_PpwpN83pI545z1OffwkWE-0Q_XITeB8Ngqd-jhBu5W-OtiemWHyywIrOUOxjFh6s2voMuDbMTtHwsmg_5yBkTynl0uEliha50f946AhKOnj3oBp6xfywkrrPm8Ct9NFYUA60HimtSBsU1dWzTU6GEzYe2Z6ZnW1DOIqPULjq0ybU1Zsfj4Bed8lUSPKHNHvOgMLE5fch7A3XD09_bBfXB_73N7befPy_Xuzvf2xlZ3Zam79LX9c7uzu9pd3my__9D5stnZXWu_Wm6_WW2_-9Tde93Zerv_41tnfWX_10bn49ZlZ7yRjMdjtWq_mFqTeV4NcqHSzQLezAIpCp55RRZmzTylZcYEzXJRZMLjheSuNh1nKa7dpKUnaVFKSLOuOIOtmVZx1RnJBeVNCcoyyDfzkKcy44FXiLLIc1YGxZAz3DPG5FNTFgifkA1xv2Mx5NyqzDNZTRYvJo8MqWsnERp2ziFtlnyvO4Ozz-eKG5AEzWY39Uj8C5uQAM8 |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Wnt%2F%CE%B2-catenin%E4%BF%A1%E5%8F%B7%E9%80%9A%E8%B7%AF%E5%9C%A8%E8%84%82%E8%82%AA%E5%B9%B2%E7%BB%86%E8%83%9E%E5%90%91%E7%A5%9E%E7%BB%8F%E5%85%83%E5%88%86%E5%8C%96%E8%BF%87%E7%A8%8B%E4%B8%AD%E7%9A%84%E4%BD%9C%E7%94%A8&rft.jtitle=%E8%A5%BF%E5%AE%89%E4%BA%A4%E9%80%9A%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5%EF%BC%88%E5%8C%BB%E5%AD%A6%E7%89%88%EF%BC%89&rft.au=%E5%A7%AC%E6%96%87%E6%99%A8&rft.au=%E8%92%8B%E5%A9%89%E5%A9%B7&rft.au=%E5%86%AF%E5%A8%87&rft.au=%E6%9D%8E%E8%90%8C&rft.date=2017&rft.pub=%E8%A5%BF%E5%AE%89%E4%BA%A4%E9%80%9A%E5%A4%A7%E5%AD%A6%E7%AC%AC%E4%B8%80%E9%99%84%E5%B1%9E%E5%8C%BB%E9%99%A2%E9%AA%A8%E7%A7%91%2C%E9%99%95%E8%A5%BF%E8%A5%BF%E5%AE%89%2C710061%25%E8%A5%BF%E5%AE%89%E5%B8%82%E7%AC%AC%E5%9B%9B%E5%8C%BB%E9%99%A2%E8%B6%85%E5%A3%B0%E8%AF%8A%E6%96%AD%E7%A7%91%2C%E9%99%95%E8%A5%BF%E8%A5%BF%E5%AE%89%2C710004&rft.issn=1671-8259&rft.volume=38&rft.issue=6&rft.spage=839&rft.epage=850&rft_id=info:doi/10.7652%2Fjdyxb201706011&rft.externalDocID=xaykdxxb201706011 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90540A%2F90540A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fxaykdxxb%2Fxaykdxxb.jpg |