Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用

目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达。结果诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组。结论脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通...

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Published in西安交通大学学报（医学版） Vol. 38; no. 6; pp. 839 - 843
Main Author 姬文晨 蒋婉婷 冯娇 李萌
Format Journal Article
LanguageChinese
Published 西安交通大学第一附属医院骨科,陕西西安,710061%西安市第四医院超声诊断科,陕西西安,710004 2017
Subjects
Wnt/β-catenin信号通路
分化
神经元
脂肪干细胞
脂肪干细胞
differentiation
Wnt/β-catenin signaling pathway
Wnt/β-catenin信号通路
神经元
adipose-derived stem cell
neuron
分化
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ISSN1671-8259
DOI10.7652/jdyxb201706011

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Summary:目的探讨Wnt/β-catenin信号通路在脂肪干细胞向神经元分化过程中的作用。方法第3代脂肪干细胞分为3组：诱导组采用成神经诱导液;抑制组在成神经诱导液的基础上加入DDK-1;对照组采用普通培养液;分别培养10d,利用Real-time PCR和Western bolt检测各组NSE、β-catenin和GSK-3β的表达。结果诱导组NSE和β-catenin呈高表达,GSK-3β呈低表达;抑制组β-catenin低表达,GSK-3β高表达,NSE的表达虽然显著低于诱导组,但是高于对照组。结论脂肪干细胞向神经元分化的过程与激活Wnt信号通路有关,但Wnt信号通路不是调控ADSCs分化的唯一通路,分化过程可能受不同信号通路的共同调控。
Bibliography:JI Wen-chen1 , JIANG Wan-ting2 , FENG Jiao1 , LI Meng1 （1.Department of Orthopedics, the First Affiliated Hospital of Xi＇an Jiaotong University, Xi＇an 710061; 2 Department of Ultrasound Diagnosis, the Fourth Hospital of Xi＇an, Xi＇an 710004, China）
Wnt/β-catenin signaling pathway; adipose-derived stem cell; neuron; differentiation
Objective To investigate the role of Wnt/β-catenin signaling pathway in the process of adiposederived stem cells（ADSCs）differentiating into neurons.Methods The third generation of ADSCs were divided into three groups.Neural induction medium was used in induction group and DDK-1 was added into neural induction medium in inhibition group.Normal culture medium was used in control group.Ten days after culture,Real-time PCR and Western blot were used to detect the expressions of NSE,β-catenin and GSK-3βin each group.ResultsThe expressions of NSE andβ-catenin were high but the expression of GSK-3 was low in induction group.The expression ofβ-catenin was lower but GSK-3 was higher in inhibiti
ISSN:1671-8259
DOI:10.7652/jdyxb201706011