基于线粒体COI序列探讨安徽长江流域黄鳝群体遗传分化

探讨安徽长江流域黄鳝群体遗传结构,采用线粒体COI的DNA条形码序列研究该区6个黄鳝野生群体（当涂、无为、繁昌、贵池、怀宁和望江）遗传分化。180个样本COI片段（665 bp）中共检出52个变异位点（变异率7.8%）、40种单倍型。序列中A、T、G、C碱基平均含量分别为24.7%、28.2%、17.1%、30.0%,A＋T含量大于G＋C含量。AMOVA分析中,高达68.45%遗传变异来自群体间,说明黄鳝群体间已产生显著遗传分化。群体间系统进化树显示：6个群体形成两大类群（当涂和繁昌群体为一类群,其余群体为另一类群）,分析表明结果与各群体地理位置密切相关。同时由NCBI下载合鳃鱼科几个物种的C...

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Bibliographic Details
Published in东北农业大学学报 Vol. 47; no. 2; pp. 74 - 80
Main Author 胡玉婷 江河 潘庭双 凌俊 段国庆
Format Journal Article
LanguageChinese
Published 安徽省农业科学院水产研究所,合肥,230031 2016
Subjects
COI条形码
遗传分化
长江
黄鳝
Monopterus albus
长江
COI barcode
遗传分化
genetic differentiation
黄鳝
COI条形码
Yangtze River
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Summary:探讨安徽长江流域黄鳝群体遗传结构,采用线粒体COI的DNA条形码序列研究该区6个黄鳝野生群体（当涂、无为、繁昌、贵池、怀宁和望江）遗传分化。180个样本COI片段（665 bp）中共检出52个变异位点（变异率7.8%）、40种单倍型。序列中A、T、G、C碱基平均含量分别为24.7%、28.2%、17.1%、30.0%,A＋T含量大于G＋C含量。AMOVA分析中,高达68.45%遗传变异来自群体间,说明黄鳝群体间已产生显著遗传分化。群体间系统进化树显示：6个群体形成两大类群（当涂和繁昌群体为一类群,其余群体为另一类群）,分析表明结果与各群体地理位置密切相关。同时由NCBI下载合鳃鱼科几个物种的COI同源序列,构建系统进化树,显示黄鳝与同属的山黄鳝聚为一支,并与不同属的合鳃鱼和穴栖蛇胸鳝聚为一支。研究表明,基于COI的DNA条形码序列适于黄鳝群体遗传结构研究。
Bibliography:Monopterus albus; COI barcode; genetic differentiation; Yangtze River
Hu Yuting,JIANG He,PAN Tingshuang,LING Jun,DUAN Guoqing（Fisheries Research Institute of Anhui Academy of Agricultural Sciences, Hefei 230031, China）
In the study, partial sequences of the mitochondrial DNA cytochrome c oxidase subunit I gene（COI） of six Monopterus albus populations, which were collected from Yangtze River basin in Anhui Province, were amplified by PCR to explore genetic differentiation. The length of COI sequences of 180 M. albus individuals were all 665 bp, 40 haplotypes were identified and 52 mutation sites were detected. The nucleotide composition of A, T, G, C was 24.7%, 28.2%, 17.1%, 30.0%, respectively; A＋T content was higher than G＋C content. Analysis of Molecular Variance（AMOVA） indicated significant genetic differentiation among populations. The six populations could be divided into two groups based on the genetic distance and phylogenetic analysis of COI sequences, which were closely related to geographical location
ISSN:1005-9369