Large contribution of human papillomavirus in vaginal neoplastic lesions: A worldwide study in 597 samples

• Published in: European journal of cancer (1990) Vol. 50; no. 16; pp. 2846 - 2854
• Main Authors: Alemany, L, Saunier, M, Tinoco, L, Quirós, B, Alvarado-Cabrero, I, Alejo, M, Joura, E.A, Maldonado, P, Klaustermeier, J, Salmerón, J, Bergeron, C, Petry, K.U, Guimerà, N, Clavero, O, Murillo, R, Clavel, C, Wain, V, Geraets, D.T, Jach, R, Cross, P, Carrilho, C, Molina, C, Shin, H.R, Mandys, V, Nowakowski, A.M, Vidal, A, Lombardi, L, Kitchener, H, Sica, A.R, Magaña-León, C, Pawlita, M, Quint, W, Bravo, I.G, Muñoz, N, de Sanjosé, S, Bosch, F.X
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2014; Elsevier
• Subjects: Aged; Biological and medical sciences; Carcinoma, Squamous Cell - complications; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - virology; Cross-Sectional Studies; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; DNA, Viral - analysis; Female; Hematology, Oncology and Palliative Medicine; Human papillomavirus; Human papillomavirus 16; Human papillomavirus 16 - isolation & purification; Humans; Immunoenzyme Techniques; International Cooperation; Medical sciences; Middle Aged; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Papillomaviridae - isolation & purification; Papillomavirus Infections - complications; Papillomavirus Infections - epidemiology; Papillomavirus Infections - virology; Pharmacology. Drug treatments; Poisson Distribution; Precancerous Conditions - epidemiology; Precancerous Conditions - virology; Prevalence; Regression Analysis; Retrospective Studies; Treatment Outcome; Tumors; Vaccine; Vaginal cancer; Vaginal intraepithelial neoplasia; Vaginal Neoplasms - complications; Vaginal Neoplasms - epidemiology; Vaginal Neoplasms - virology; World; Human papillomavirus; Vaccine; Vaginal intraepithelial neoplasia; Vaginal cancer; Vagina; Papovaviridae; Malignant tumor; Infection; Papillomavirus; Virus; Cancerology; Viral disease; Lesion; Intraepithelial neoplasia; Cancer
• ISSN: 0959-8049; 1879-0852
• DOI: 10.1016/j.ejca.2014.07.018

Abstract Aim This work describes the human papillomavirus (HPV) prevalence and the HPV type distribution in a large series of vaginal intraepithelial neoplasia (VAIN) grades 2/3 and vaginal cancer worldwide. Methods We analysed 189 VAIN 2/3 and 408 invasive vaginal cancer cases collected from 31 countries from 1986 to 2011. After histopathological evaluation of sectioned formalin-fixed paraffin-embedded samples, HPV DNA detection and typing was performed using the SPF-10/DNA enzyme immunoassay (DEIA)/LiPA25 system (version 1). A subset of 146 vaginal cancers was tested for p16INK4a expression, a cellular surrogate marker for HPV transformation. Prevalence ratios were estimated using multivariate Poisson regression with robust variance. Results HPV DNA was detected in 74% (95% confidence interval (CI): 70–78%) of invasive cancers and in 96% (95% CI: 92–98%) of VAIN 2/3. Among cancers, the highest detection rates were observed in warty-basaloid subtype of squamous cell carcinomas, and in younger ages. Concerning the type-specific distribution, HPV16 was the most frequently type detected in both precancerous and cancerous lesions (59%). p16INK4a overexpression was found in 87% of HPV DNA positive vaginal cancer cases. Conclusions HPV was identified in a large proportion of invasive vaginal cancers and in almost all VAIN 2/3. HPV16 was the most common type detected. A large impact in the reduction of the burden of vaginal neoplastic lesions is expected among vaccinated cohorts.