ISOLATION ON TREHALAMINE, THE AGLYCON OF TREHAZOLIN, FROM MICROBIAL BROTHS AND CHARACTERIZATION OF TREHAZOLIN RELATED COMPOUNDS

• Published in: Journal of antibiotics Vol. 46; no. 7; pp. 1116 - 1125
• Main Authors: ANDO, OSAMU, NAKAJIMA, MUTSUO, HAMANO, KIYOSHI, ITOI, KAZUKO, TAKAHASHI, SHUJI, TAKAMATSU, YASUYUKI, SATO, AKIRA, ENOKITA, RYUZO, OKAZAKI, TAKAO, HARUYAMA, HIDEYUKI, KINOSHITA, TAKESHI
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1993; Japan Antibiotics Research Association
• Subjects: Actinobacteria - classification; Actinobacteria - metabolism; Animals; Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Applied microbiology; Biological and medical sciences; Bombyx; Chemotherapic agents; Disaccharides - metabolism; Fundamental and applied biological sciences. Psychology; Glycoside Hydrolases - antagonists & inhibitors; Hydrolysis; Microbiology; Micromonospora - classification; Micromonospora - metabolism; Molecular Structure; Nuts; Oxazoles - chemistry; Oxazoles - isolation & purification; Oxazoles - pharmacology; Rats; β-D-Glucosidase; Molecular structure; Enzyme; Micromonosporaceae; Micromonospora; Aglycone; Amycolatopsis; Enzyme inhibitor; Pseudonocardiaceae; Actinomycetales; Sucrose α-D-glucohydrolase; Microbial origin; Bacteria; Isolation; Actinomycetes; Physicochemical properties
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.46.1116

Trehalamine, (3aR, 4R, 5S, 6S, 6aS)-2-amino-4-(hydroxymethyl)-3a, 5, 6, 6a-tetrahydro-4H-cyclopent[d]oxazole-4, 5, 6-triol (1) and D-glucose were obtained by acid hydrolysis of trehazolin (3), a trehalase inhibitor produced by actinomycetes. More vigorous hydrolytic treatment of trehazolin afforded an aminocyclitol, (1R, 2S, 3R, 4S, 5R)-5-ammo-l-(hydroxymethyl)cyclopentane-1, 2, 3, 4-tetraol (2). Trehalamine, the aglycon of trehazolin, was also found in the culture broths of two trehazolin producing strains, Micromonospora sp. SANK 62390 and Amycolatopsis sp. SANK 60791. These trehazolin related compounds trehalamine (1) and 2 were poor inhibitors of trehalase (1; IC50 1.8 × 10-4 M, 2; > 5.0 × 10-4 M). On the other hand they inhibited more potently rat intestinal sucrase (1; IC50 6.8 × 10-5M) and sweet almond β-glucosidase (2; IC50 5.6× 10-6M) than trehazolin.