Display of the antigenic region of Nipah virus nucleocapsid protein on hepatitis B virus capsid

• Published in: Journal of bioscience and bioengineering Vol. 113; no. 1; pp. 26 - 29
• Main Authors: Yap, Wei Boon, Tey, Beng Ti, Mohamed Alitheen, Noorjahan Banu, Tan, Wen Siang
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2012; Elsevier
• Subjects: Animals; antigens; Antigens, Viral - immunology; Antisera; antiserum; Biological and medical sciences; Biotechnology; capsid; Capsid - metabolism; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; hepatitis; Hepatitis B Antibodies - immunology; hepatitis B antigens; Hepatitis B core antigen; Hepatitis B Core Antigens - immunology; Hepatitis B virus; Hepatitis B virus - metabolism; Humans; Nipah virus; Nipah Virus - immunology; Nucleocapsid protein; nucleocapsid proteins; Nucleocapsid Proteins - immunology; Recombinant Proteins - immunology; Swine; Virus-like particles; Nipah virus; Hepatitis B virus; Hepatitis B core antigen; Nucleocapsid protein; Virus-like particles; Virus; Hepadnaviridae; Virus like particle; Nucleocapsid; Orthohepadnavirus; Capsid; Protein
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2011.09.007

The C-terminal domain of Nipah virus (NiV) nucleocapsid protein (NP 401–532) was inserted at the N-terminus and the immunodominant loop of hepatitis B core antigen (HBc). The stability of NP 401–532 increased tremendously when displayed on the HBc particles. These particles reacted specifically with the swine anti-NiV and the human anti-HBc antisera.