Characteristics of Bone Strength and Metabolism in Type 2 Diabetic Model Nagoya Shibata Yasuda Mice

• Published in: Biological & pharmaceutical bulletin Vol. 41; no. 10; pp. 1567 - 1573
• Main Authors: Tanaka, Hiroaki, Miura, Toshihiro, Yamashita, Takenori, Yoneda, Misao, Takagi, Satoshi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.10.2018; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Animal models; Animals; Blood Glucose - genetics; Blood Glucose - metabolism; Body weight; Bone Density - physiology; Bone mass; bone metabolism; bone microstructure; Bone mineral density; Bone strength; Bone turnover; Cancellous bone; Cortical bone; Diabetes mellitus; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - metabolism; Diabetes Mellitus, Type 2 - pathology; Disease Models, Animal; Femur; Femur - metabolism; Femur - pathology; Glucose; Glucose metabolism; Glucose tolerance; Hyperglycemia; Insulin; Male; Metabolism; Mice; Mice, Inbred ICR; Mice, Transgenic; Microstructure; Nagoya Shibata Yasuda mouse; Turnover rate; type 2 diabetes mellitus; type 2 diabetes mellitus; bone mass; Nagoya Shibata Yasuda mouse; bone metabolism; bone strength; bone microstructure
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b18-00275

We evaluated the suitability of Nagoya Shibata Yasuda (NSY) mice as an animal model for examining the influence of a glucose metabolism disorder on bone integrity, using Institute of Cancer Research (ICR) mice as controls. We selected six NSY and ICR mice each that were matched for weight, and measured serum glucose levels, serum insulin levels, and conducted an oral glucose tolerance test. Histological sections of the femurs of both mouse lines were prepared, and the bone strength, mass, and microstructure of the femur were compared, along with bone metabolism. Serum glucose levels were significantly higher in the NSY mice than in the control mice, but body weight and serum insulin levels did not differ between the groups. Bone mass, microstructure, and strength of the femur, and bone metabolism were lower in the NSY mice than in the control mice. In the cortical bone of the femur in the NSY mice, several parts were not stained with eosin, demonstrating a strong negative correlation between serum glucose levels and bone mineral density; however, there was a negative correlation between serum glucose levels and bone metabolic markers. The bone turnover rate in the NSY mice was decreased by hyperglycemia, resulting in a thinner and shorter femur, reduced cortical and trabecular areas, and lower bone mass compared to those of the control mice. Collectively, these results suggest deteriorated bone strength of the femur in NSY mice, serving as a useful model for studying the link between glucose metabolism and bone integrity.