Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration

Published selective media were evaluated for the isolation of Aeromonas spp. from environmental samples by membrane filtration. Satisfactory recoveries were obtained only with mA agar (Rippey & Cabelli) and dextrin-fuchsin-sulphite agar (Schubert), but neither was sufficiently selective. The pos...

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Bibliographic Details
Published inJournal of applied bacteriology Vol. 62; no. 3; pp. 279 - 287
Main Authors Havelaar, A.H, During, M, Versteegh, J.F.M
Format Journal Article
LanguageEnglish
Published England 01.03.1987
Subjects
Aeromonas
Aeromonas - growth & development
Aeromonas - isolation & purification
Ampicillin
Culture Media
Dextrins
Filtration
Fresh Water
membranes
pathogens
plate count
Seawater
Sewage
Water Microbiology
water pollution
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Summary:Published selective media were evaluated for the isolation of Aeromonas spp. from environmental samples by membrane filtration. Satisfactory recoveries were obtained only with mA agar (Rippey & Cabelli) and dextrin-fuchsin-sulphite agar (Schubert), but neither was sufficiently selective. The positive aspects of these two media were combined in a new medium, ampicillin-dextrin agar. Recovery from pure cultures and environmental samples was optimal at an ampicillin concentration of 10 mg/l and incubation for 24 h at 30 degrees C under aerobic conditions, and specificity was high (i.e. confirmation rate usually greater than 90%, no false negative colonies encountered). The medium can also be used for isolation of Aeromonas spp. from sea water provided that the vibriostatic agent 0/129 is added at 50 mg/l.
ISSN:0021-8847
2056-5232
DOI:10.1111/j.1365-2672.1987.tb02410.x