Crystallization and preliminary X-ray analysis of a class II release factor RF3 from a sulfate-reducing bacterium

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 64; no. 7; pp. 622 - 624
• Main Authors: Kihira, Kiyohito, Numata, Shuko, Kitamura, Masaya, Kondo, Jun, Terawaki, Shinichi, Shomura, Yasuhito, Komori, Hirofumi, Shibata, Naoki, Higuchi, Yoshiki
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.07.2008
• Subjects: Bacteria; Bacterial Proteins - chemistry; Bacterial Proteins - classification; Bacterial Proteins - genetics; CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; Crystallization Communications; Desulfovibrio vulgaris; Desulfovibrio vulgaris - chemistry; Desulfovibrio vulgaris - genetics; DIFFUSION; DISSOCIATION; Gene Expression Regulation, Bacterial; GTP-binding proteins; MOLECULES; MONOCRYSTALS; Peptide Termination Factors - chemistry; Peptide Termination Factors - classification; Peptide Termination Factors - genetics; RESOLUTION; SOLVENTS; SPACE GROUPS; Sulfates - chemistry; Sulfates - metabolism; SYNCHROTRON RADIATION; translation factors; X-Ray Diffraction
• ISSN: 1744-3091; 1744-3091
• DOI: 10.1107/S1744309108015583

Class II release factor 3 (RF3) from the sulfate‐reducing bacterium Desulfovibrio vulgaris Miyazaki F, which promotes rapid dissociation of a class I release factor, has been overexpressed, purified and crystallized in complex with GDP at 293 K using the sitting‐drop vapour‐diffusion method. A data set was collected to 1.8 Å resolution from a single crystal at 100 K using synchrotron radiation. The crystal belongs to space group P1, with unit‐cell parameters a = 47.39, b = 82.80, c = 148.29 Å, α = 104.21, β = 89.78, γ = 89.63°. The asymmetric unit contains four molecules of the RF3–GDP complex. The Matthews coefficient was calculated to be 2.3 Å3 Da−1 and the solvent content was estimated to be 46.6%.