大鼠骨骼椎间盘累积损伤性炎症反应中大麻素受体的表达及意义的初步研究

目的：通过检测大麻素（CB）1、2型受体（CB－1R、CB－2R）在大鼠累积损伤性椎间盘炎症反应过程中的表达来探讨其中大麻素的参与过程及作用机制。方法清洁级Wistar大鼠随机分成实验组与对照组，根据Jill A．Ulrich制作方法准备实验组，以切皮后即缝合为对照组，在术后第3、7、10 d，实验组与对照组每次各取6只，取出相应椎间盘，进行形态学观察，应用RealTime－PCR技术检测CB－1R、CB－2R的表达水平；利用Western Blot方法检测CB－2R蛋白含量。结果与对照组相比，实验组10 d可见刺伤所致纤维环断裂，髓核内出血。在椎间盘累积性损伤后，CB－1R、CB－2R呈时间...

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Published in中国骨质疏松杂志 Vol. 21; no. 4; pp. 429 - 433
Main Author 梁峰 刘振宁 谭树森 贾长青 柏树令
Format Journal Article
LanguageChinese
Published 中国医科大学附属盛京医院脊柱关节骨科,沈阳,110004 2015
Subjects
大麻素受体
累积性损伤
骨骼椎间盘炎症反应
大麻素受体
Bone and disc inflammation
Cannabinoids receptor
Cumulative damage
骨骼椎间盘炎症反应
累积性损伤
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ISSN1006-7108

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Summary:目的：通过检测大麻素（CB）1、2型受体（CB－1R、CB－2R）在大鼠累积损伤性椎间盘炎症反应过程中的表达来探讨其中大麻素的参与过程及作用机制。方法清洁级Wistar大鼠随机分成实验组与对照组，根据Jill A．Ulrich制作方法准备实验组，以切皮后即缝合为对照组，在术后第3、7、10 d，实验组与对照组每次各取6只，取出相应椎间盘，进行形态学观察，应用RealTime－PCR技术检测CB－1R、CB－2R的表达水平；利用Western Blot方法检测CB－2R蛋白含量。结果与对照组相比，实验组10 d可见刺伤所致纤维环断裂，髓核内出血。在椎间盘累积性损伤后，CB－1R、CB－2R呈时间依赖性增高趋势。3、7、10 d组CB－2R蛋白表达与对照组相比明显增高（ P＜0．01）。结论在骨骼椎间盘反复损伤及其后炎症反应阶段，CB类物质参与了炎症反应的发展过程，从而抑制骨骼椎间盘的退变。
Bibliography:Objective To reveal the function of CB during disc inflammation due to cumulative damage by detecting CB-1R and CB-2R.Methods Wistar rats were randomly divided into experiment group and control group.The experiment group was prepared according to Jill A Ulrich method.Control group was prepared by skin cutting and suturing.Six rats were taken from each group on 3rd day, 7th day, and 10th day after the operation.The intervertebral discs were collected.Morphology was observed. Expression of CB-1R and CB-2R was detected using real-time PCR.CB-2R protein content was examined using Western blotting. Results Compared with the control group, the annulus rupture and nucleus bleeding were observed in experiment group in 10 days.The expression levels of CB receptor increased significantly in a time-dependent manner.CB-2R protein expression increased more significantly at the 3rd day, 7th day, and 10th day in experiment group than that in control group （P〈0.01）.Conclusion CB is involved in the regulation of the rat bone
ISSN:1006-7108