芦荟苷A、B以及异芦荟色苷D的同时分离纯化

首次采用反相中压制备色谱从芦荟中一次分离制备得到芦荟苷A、B以及异芦荟色苷D。以库拉索芦荟丙酮粗提物为原料，采用中压制备色谱系统：SCO色谱柱（40cm×26cm，30～50μm），流动相甲醇-0．5％乙酸水（33：67，V／V），流速20mL／min，等度洗脱方式，柱温室温，检测波长254Bin，收集波长356nm对芦荟样品进行分离制备，得到三种化合物单体。经高效液相色谱、紫外、红外、质谱及核磁共振等方法分析表明所得到的三种化合物分别是异芦荟色苷D、芦荟苷A和芦荟苷B，其纯度分别达到了98．0％，96．0％和98．9％。该方法简便，产品质量高，一次制备可以得到多种单体，为芦荟成分的测定与药理...

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Published in天然产物研究与开发 Vol. 23; no. 5; pp. 878 - 881
Main Author 李婷 钟英 王芝 万金志
Format Journal Article
LanguageChinese
Published 中山大学药学院药物分析实验室,广州,510006%中国农村技术开发中心,北京,100045 2011
Subjects
B
中压制备色谱
异芦荟色苷D
芦荟苷A
异芦荟色苷D
芦荟苷A、B
中压制备色谱
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Summary:首次采用反相中压制备色谱从芦荟中一次分离制备得到芦荟苷A、B以及异芦荟色苷D。以库拉索芦荟丙酮粗提物为原料，采用中压制备色谱系统：SCO色谱柱（40cm×26cm，30～50μm），流动相甲醇-0．5％乙酸水（33：67，V／V），流速20mL／min，等度洗脱方式，柱温室温，检测波长254Bin，收集波长356nm对芦荟样品进行分离制备，得到三种化合物单体。经高效液相色谱、紫外、红外、质谱及核磁共振等方法分析表明所得到的三种化合物分别是异芦荟色苷D、芦荟苷A和芦荟苷B，其纯度分别达到了98．0％，96．0％和98．9％。该方法简便，产品质量高，一次制备可以得到多种单体，为芦荟成分的测定与药理活性的研究提供了条件。
Bibliography:A medium pressure preparative liquid chromatography （MPLC） was used to isolate the aloins A and B and isoaloeresin D from Aloe vera simultaneously for the first time. The crude material extracted by acetone, was performed with ODS column （40 cm ×26 cm,30-50 μm） by using isoeratie elution with methanol-0.5% acetic water （33：67 ,v/ v） as the mobile phase at a flow rate of 20 mL/min. The detecting and collecting wavelengths were set at 254 nm and 356 nm,respectively, and the column temperature was room temperature. Three compounds were identified as aloins A and B and isoaloeresin D by using UV,IR,MS and NMR,and their purities were 98.0% ,96.0% and 98.9% ,respectively. The method was convenient, and active constituents with high purity could be obtained simultaneously,which provided conditions to investigate the constituents and their activities of Aloe vera.
LI Ting ,ZHONG Ying ,WANG Zhi ,WAN Jin-zhi 1Laboratory for Pharmaceutical Analysis and Quality Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen Un
ISSN:1001-6880
DOI:10.3969/j.issn.1001-6880.2011.05.020