沉默生物钟基因PER1对口腔鳞状细胞癌细胞中生物钟基因网络的影响

目的探讨口腔鳞状细胞癌细胞中生物钟基因PER1对生物钟基因网络中其他生物钟基因表达的影响。方法采用RNA干扰技术沉默人口腔鳞状细胞癌SCC15细胞中PER1基因，应用流式细胞仪检测沉默后细胞的增殖和凋亡水平，实时荧光定量聚合酶链式反应检测生物钟基因CLOCK、BMAL1、PER1、PER2、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIE、RORA、NPAS2、REV．ERBAmRNA的表达情况。结果PER1基因沉默后，SCC15细胞增殖指数上升，凋亡指数下降（P〈0．05）；PER1、PER2、DEC1、DEC2、CRY1、CRY2和NPAS2 mRNA的表达水平降低（P〈...

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Bibliographic Details
Published in华西口腔医学杂志 Vol. 35; no. 1; pp. 57 - 62
Main Author 赵钦 敖奕然 杨凯 苏小丽 吕晓强
Format Journal Article
LanguageChinese
Published 重庆医科大学附属第一医院口腔颌面外科,重庆,400016 2017
Subjects
口腔
基因
生物钟
鳞状细胞癌
生物钟
gene
鳞状细胞癌
oral cavity
口腔
基因
circadian clock
squamous cell carcinoma
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Summary:目的探讨口腔鳞状细胞癌细胞中生物钟基因PER1对生物钟基因网络中其他生物钟基因表达的影响。方法采用RNA干扰技术沉默人口腔鳞状细胞癌SCC15细胞中PER1基因，应用流式细胞仪检测沉默后细胞的增殖和凋亡水平，实时荧光定量聚合酶链式反应检测生物钟基因CLOCK、BMAL1、PER1、PER2、PER3、DEC1、DEC2、CRY1、CRY2、TIM、CKIE、RORA、NPAS2、REV．ERBAmRNA的表达情况。结果PER1基因沉默后，SCC15细胞增殖指数上升，凋亡指数下降（P〈0．05）；PER1、PER2、DEC1、DEC2、CRY1、CRY2和NPAS2 mRNA的表达水平降低（P〈0．05），PER3、TIM、RORA和REV—ERBA mRNA的表达水平升高（P〈0．05），CLOCK、BMAL1和CKIE mRNA的表达水平无统计学改变（P〉0．05）。结论生物钟基因PER1能够调控生物钟基因网络中众多其他生物钟基因PER2、DEC1、DEC2、CRY1、CRY2、NPAS2、PER3、TIM、RORA和REV—ERBA的表达，PER1在生物钟基因网络中具有重要调控作用。
Bibliography:51-1169/R
Zhao Qin, Ao Yiran, Fang Kai, Su Xiaoli, Lu Xiaoqiang. （Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China）
circadian clock; squamous cell carcinoma; oral cavity; gene
Objective This study investigated the effect of clock gene PER1 on the expression levels of other clock genes in clock gene networks in oral squamous cell carcinoma cells. Methods We used RNA interference mediated by short hairpin RNAs （shRNAs） to effectively knock down PER1 in SCC15 human oral squamous cell carcinoma cells. Flow cytometry was used to detect the degree of proliferation and apoptosis of the cells after PER1 knockdown, and quantitative real-time PCR was used to detect the mRNA expression levels of the clock genes CLOCK, BMAL1, PER1, PER2, PER3, DEC1, DEC2, CRY1, CRY2, TIM, CKIE, RORA, NPAS2, and REV-ERBA. Results The proliferation index of SCC15 cells increased significantly while the apoptotic index decreased significantly after PER1 knockdown （P
ISSN:1000-1182
DOI:10.7518/hxkq.2017.01.008