Excystment-Dependent Alteration of Protein Expression in Terrestrial Ciliate Colpoda cucullus

• Published in: Microbes and Environments Vol. 28; no. 3; pp. 388 - 390
• Main Authors: Sogame, Yoichiro, Kojima, Katsuhiko, Takeshita, Toshikazu, Kinoshita, Eiji, Funadani, Ryoji, Matsuoka, Tatsuomi
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2013; Japan Science and Technology Agency; Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology
• Subjects: Amino Acid Sequence; Ciliophora - genetics; Ciliophora - growth & development; Ciliophora - isolation & purification; Ciliophora - metabolism; Colpoda; DEAD-box RNA helicase; Electrophoresis, Gel, Two-Dimensional; excystment; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Short Communication; Soil - parasitology
• ISSN: 1342-6311; 1347-4405
• DOI: 10.1264/jsme2.ME12200

Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis.